猪β_2-肾上腺素能受体初级克隆质粒的改造与鉴定  

Modification and identification of the primary β_2-adrenergic receptor gene cloned plasmid

作　　者：陈勇[1] 周光宏[1] 刘清[1] 吉传义[2] 

机构地区：[1]南京农业大学动物科技学院,江苏南京210095 [2]南京农业大学动物医学院

出　　处：《畜牧与兽医》2000年第4期3-5,共3页Animal Husbandry & Veterinary Medicine

基　　金：江苏省科委 2 1世纪农业新科技革命资助项目! (BG9850 1 15)

摘　　要：猪 β2 肾上腺素能受体 (beta2 adrenergicreceptor,β2 AR)基因初级克隆质粒 (pMDAR)在 β2 AR基因 5′端外侧含有 2个EcoRⅠ酶切位点。用EcoRⅠ酶切消化和T4DNA连接酶连接后 ,获得了具有EcoRⅠ单一酶切位点的改造质粒。对经过修饰后的质粒进行了多酶切和 β2 AR基因片段回切等方法鉴定。This research deals with the modification and identification of the primary β 2 adrenergic receptor gene cloned plasmid pMDAR.Two cut sites of endonuclease EcoR Ⅰ was located into 5′ terminal of β 2 AR gene.After being digested with EcoR Ⅰ,the recombinant plasmid with unique EcoR Ⅰ site was produced.By restriction enzyme characterization,the new recombinant plasmid pMDAR/E was determined.

关 键 词：猪 Β2-肾上腺素能受体 初级克隆质粒 主动免疫 

分 类 号：S828.2[农业科学—畜牧学] Q78[农业科学—畜牧兽医]