乌司他丁对脓毒症膈肌p38MAPK信号通路的影响  

The effects of ulinastatin on p38 MAPK signal pathway in diaphragmatic damage caused by sepsis

作　　者：张红梅[1] 刘培[2] 盛春风[2] 郑红梅[3] 

机构地区：[1]湖北医药学院附属太和医院呼吸内科,湖北十堰442000 [2]湖北医药学院附属太和医院重症医学科,湖北十堰442000 [3]湖北医药学院附属太和医院普外科,湖北十堰442000

出　　处：《重庆医学》2013年第18期2117-2119,共3页Chongqing medicine

基　　金：十堰市科技公关基金资助项目(2009045D)

摘　　要：目的观察乌司他丁对脓毒症膈肌p38MAPK信号通路的影响,以探讨其对脓毒症膈肌损伤的保护作用。方法 SPF级成年雄性SD大鼠30只,随机分为A组(假手术组,n=10)、B组(脓毒症组,n=10)及C组(乌司他丁治疗组,n=10)。其中,A组在给予水合氯醛麻醉成功后,从正中线剪开大鼠腹部,然后缝合伤口。B组和C组在麻醉成功后,使用盲肠结扎穿刺(CLP)模型将大鼠制成脓毒症动物模型,并且C组在术后立即给予乌司他丁治疗。所有大鼠在术后16h处死,取左侧膈肌检测肌条收缩力,右侧膈肌检测膈肌肌细胞凋亡率及p38MAPK蛋白表达水平。结果脓毒症组与假手术组比较,膈肌肌细胞凋亡率及p38MAPK蛋白表达明显上升,肌条收缩力明显下降,两组比较差异有统计学意义(P<0.01)。给予乌司他丁治疗后,膈肌细胞凋亡率、p38MAPK蛋白表达及肌条收缩力较脓毒症组明显恢复,两组比较差异有统计学意义(P<0.01)。结论乌司他丁能够有效抑制脓毒症时p38MAPK蛋白的表达,减少膈肌肌细胞凋亡率,从而减轻脓毒症膈肌损伤。Objective In order to explore the protective effects of ulinastatin against diaphragmatic damage caused by sepsis,we investigated the effects of ulinastatin on the p38 MAPK protein expression in the diaphragm.Methods Thirty SD rats were randomly divided into three groups：Group A（Sham-operated group,n=10）,Group B（Sepsis group,n=10） and Group C（ulinastatin-treated group,n=10）.The rats of Group A were only cut from the abdomen,and then sutured the wound after the anesthetic.The rats of Group B and C were made into Cecal ligation and puncture model（CLP）,and then the rats of Group C were treated with ulinastatin.All rats were killed at 16 hours after operations,taken the left side of diaphragm muscle to detect the contractile force and taken the right side of diaphragm muscle to detect the diaphragmatic muscle cell apoptosis rate and the p38 MAPK protein expression.Results Group B compared with Group A,diaphragm contractility significant drop,the rate of diaphragm muscle cell apoptosis,the number of p38 MAPK protein expression was significantly increased,the two groups was significant difference（P＆lt;0.01）.After the ulinastatin therapy,the diaphragmatic muscle cell apoptosis rate,the number of p38 MAPK protein expression and the contractile force were resumed obviously.Conclusion Ulinastatin can effectively inhibit sepsis p38 MAPK protein expression,reduce the rate of diaphragm muscle cell apoptosis,thereby reducing sepsis diaphragmatic injury.

关 键 词：脓毒症 细胞凋亡 乌司他丁 

分 类 号：R459.7[医药卫生—急诊医学]