连翘酯苷对小鼠不同组织巨噬细胞功能的影响  

Effects of Forsythoside on the Functions of Different Tissue Phagocytes from Mice

作　　者：张永红[1] 芦山[1] 陈舒楠[1] 柴丽娜[1] 沈红[1] 崔德凤[1] 

机构地区：[1]北京农学院动物科学技术学院,北京102206

出　　处：《中国农学通报》2013年第17期32-36,共5页Chinese Agricultural Science Bulletin

基　　金：北京市教委项目"抗菌中兽药成分组方原则及新兽药研发"(PXM2012_014207_000013);"酶抑制法检测辛辣蔬菜农药残毒时假阳性问题的研究"(KM200810020014)

摘　　要：探讨连翘酯苷(FS)对小鼠不同组织巨噬细胞(Mφ)功能的影响。不同组织Mφ体外培养在添加FS和脂多糖(LPS)的培养基中,通过MTT、ELISA和Griess、瑞氏染色检测细胞增值、分泌和吞噬能力。FS组腹腔Mφ与LPS+FS组骨髓Mφ吸光度A值极显著提高,但FS组脾脏和骨髓Mφ以及LPS+FS组腹腔和脾脏MφA值降低明显;FS显著提高环磷酰胺(CY)处理鼠脾脏和骨髓Mφ增值但明显抑制腹腔Mφ增殖。FS显著抑制Mφ分泌肿瘤坏死因子-α(TNF-α);FS明显促进LPS刺激的腹腔MφNO分泌,但脾脏MφNO分泌量明显减少,且有显著剂量依赖性;FS明显抑制CY处理鼠脾脏和骨髓Mφ分泌NO。FS促进LPS刺激的腹腔Mφ吞噬鸡红细胞能力。FS对小鼠不同组织Mφ增殖、TNF-α和NO分泌以及吞噬能力都有影响,这可能是FS调节细胞免疫功能的机制之一。The objective was to investigate effects of forsythoside （FS） on the function of different tissue macrophages from the mice. Different tissue macrophages were in vitro cultured in media containing FS and lipopolysaccharide （LPS）. Cell proliferation, secretion and phagocytic ability of macrophages were detected by MTT, ELISA, Griess and Wright-Giemsa staining method. Compared with LPS group, it was very significantly increased in absorbance values of peritoneal macrophage treated with FS and of bone marrow macrophages stimulated with FS＋LPS, but decreased markedly in those of spleen and bone marrow macrophages treated with FS and of peritoneal and spleen nmcrophages with FS ＋ LPS. FS significantly improved cell proliferation of spleen and bone marrow macrophages while significantly inhibited that of peritoneal macrophages from mice treated with cyclophosphamide （CY）. FS significantly decreased TNF-a secretion level of macrophages. FS significantly increased NO secretion level of LPS-stimulated peritoneal macrophages and reduced that of spleen macrophages and also there was a significant dose-dependence. FS significantly inhibited IN（） secretion levels of spleen and bone marrow macrophages from CY-treated mice but did not affect peritoneal macrophages. FS could enhance the phagocytosis of LPS-stimulated peritoneal macrophages to chicken red blood cells. FS had an impact on cell proliferation of different tissue macrophages, TNF-a and NO secretion, and phagocytic ability, which might be one of FS mechanism to regulate cellular immune function.

关 键 词：连翘酯苷 小鼠 巨噬细胞 增殖 分泌 吞噬作用 

分 类 号：S853.74[农业科学—临床兽医学]