响应面法优化巨大芽胞杆菌发酵培养基  

Optimization of Medium for Bacillus megaterium Fermentation by Response-surface Methodology

作　　者：刘清术[1] 郭照辉[1] 刘前刚[1] 吴民熙[1] 陈海荣[1] 

机构地区：[1]湖南省微生物研究所/湖南省农用微生物应用工程技术研究中心,长沙410009

出　　处：《中国农学通报》2013年第18期142-146,共5页Chinese Agricultural Science Bulletin

基　　金：湖南省农业科技计划项目"烟草生物有机药肥的研制";湖南省"一化四体系"专项资金项目"稻田用生物菌肥的研制"

摘　　要：为了提高微生物肥料生产菌株——巨大芽胞杆菌1013的发酵产芽孢数量,采用响应面法对其发酵培养基进行优化。首先采用单因子试验筛选出最佳碳源和氮源;然后设计基础培养基,采用Plackett-Burman方法对基础培养基中影响发酵产芽胞数各因素的效应进行评价,筛选出有显著效应的因素;最后通过响应面分析法优化主要因素的最佳浓度。结果表明,最佳碳源为糖蜜,最佳氮源豆饼粉和硝酸铵;基础培养基中对产芽孢数有显著效应的因素为糖蜜和豆饼粉,响应面优化后这2个因素的最佳含量为:糖蜜4.17%,豆饼粉1.53%。采用优化后的培养基进行摇瓶发酵,巨大芽胞杆菌的发酵产芽胞数为(89.34±3.87)×108cfu/mL,比基础培养基提高了3.35倍。The response surface methodology was used to optimize the fermentation medium of Bacillus megaterium 1013. In the single factor experiment, molasses, soybean powder and NH4NO3, were selected to be the optimal carbon source and nitrogen source, respectively. Then, molasses and soybean powder were found to be the important factors by Plackett-Burman design. The optimal concentration of two important factors was determined by response surface methodology. The results showed that, 4.17% molasses, 1.53% soybean powder. The spores yield of Bacillus megaterium 1013 could reach （89.34±3.87） × 108 cfu/mL by using the optimal medium, which was increased by 3.35 fold compared with the original medium.

关 键 词：响应面设计法 巨大芽胞杆菌 培养基优化 

分 类 号：Q939.96[生物学—微生物学]