天南星超临界CO_2乙醇萃取物抗惊厥作用的实验研究  

Anticonvulsant Effects of Supercritical-CO_2 Alcohol Fluid Extractions of Rhizoma Pinelliae

作　　者：杨蓉[1] 王明正[2] 成银霞[2] 

机构地区：[1]山西中医学院 [2]山西医科大学

出　　处：《中西医结合心脑血管病杂志》2013年第6期736-738,共3页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：山西省自然科学基金资助项目(No.20001065)

摘　　要：目的研究中草药天南星超临界CO2乙醇萃取物对不同惊厥动物模型的对抗作用,并探讨其作用机制。方法采用最大电休克(MES)和戊四唑惊厥模型(MET),以托吡酯(TPM)为阳性对照,观察其抗惊厥作用;建立大鼠皮层定位注射青霉素点燃模型,观察其灌胃给药对惊厥行为和脑电图的影响。结果天南星超临界CO2萃取物对MES模型有对抗作用,且量效呈正相关性。可延长MET惊厥潜伏期(P<0.01),对抗MET发作。对青霉素点燃模型亦产生对抗作用,可延长痫性发作潜伏期、减轻发作程度,延长痫性放电的潜伏期,减少痫波发放频率,减小放电最高波幅,与模型组比较,差异有统计学意义(P<0.05或P<0.01)。结论天南星超临界CO2乙醇萃取物可剂量依赖性对抗小鼠最大电休克惊厥,对抗戊四唑惊厥,也能对抗大鼠皮层定位注射青霉素点燃发作,其抗惊厥作用性质和TPM相似。Objective To investigate the antagonize action of supercritical - CO2 alcohol fluid extraction of rhizoma pinelliae（SFE - CO2 RP） on different convulsive model and explore its mechanism of anticonvulsant action. Method Maximal electroshock seizure （MES） and Metrazol seizure test （MET） were used to analyze the anticonvulsant effects in mice treated by SFE - CO2 RP. The model of seizure rats induced hy penicil.lin injected in cortex was used to investigate effect of SFE - CO2 RP on the convulsant behaviors and hippocampus EEG. Result SFE - CO2 RP was dose - dependently antagonize MES,and could prolong latent period （P（0.01） on MET in mice, and could also prolong the latent period on the penicillin- induced seizure and lighten the extent of seizure. At the same time,it could prolong latent period of epileptiform discharge,reduce the frequency of epileptiform discharge,and decrease the highest wave of hippocampus EEG. All above indexes were significantly difference between model group and control groups（P〈0.05 or P〈0.01）. Conclusion SFE -CO2 RP was dose -dependently antagonize MES and antagonize MET in mice,it also could antagonize peni- cillin - kindling seizure in rats. Anticonvulsant properties of SFE - CO2 RP was resemble as TPM.

关 键 词：天南星超临界CO2乙醇萃取物 最大电休克惊厥 戊四唑惊厥 青霉素惊厥模型 

分 类 号：R285[医药卫生—中药学]