高压氧对丙戊酸钠孤独症模型鼠学习记忆能力及海马锥体细胞形态学的影响  

Effects of hyperbaric oxygen on learning and memory ability in VPA autism and on the morphology of pyramidal cells in the CA1 region of the hippocampus

作　　者：衣明纪[1] 冯雪英[1] 付伟伟[2] 李音[3] 

机构地区：[1]青岛大学医学院附属医院儿童保健科,青岛266003 [2]青岛大学医学院附属医院病理科,青岛266003 [3]青岛市妇女儿童医疗保健中心

出　　处：《中华物理医学与康复杂志》2013年第6期447-451,共5页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：基金项目：青岛大学医学院附属医院重点培育学科专项建设基金项目（2010610）

摘　　要：目的探讨高压氧干预对丙戊酸钠（VPA）孤独症模型鼠学习记忆能力及海马锥体细胞形态学的影响。方法通过Wistar大鼠怀孕第12．5天腹腔注射VPA的方法制作VPA孤独症大鼠模型，孤独症模型大鼠分为高压氧治疗组、高压空气治疗组、常压高氧治疗组、常压空气模型组，每组雄鼠12只；从正常对照组中随机选取12只雄鼠，作为常压空气正常组。孤独症模型鼠的干预措施：采用动物实验舱，高压氧治疗组每次治疗前用纯氧洗舱10min，纯氧加压15min，于2．0ATA稳压45min，减压15min；高压空气治疗组空气加压15min，于2．0ATA稳压45min，减压15min；常压高氧治疗组治疗前纯氧洗舱10min，不关紧舱门，持续供给纯氧1h；常压空气模型组每日置于开放的动物实验舱1h，不加压，不关舱门；各组每日干预1次，每次1h，疗程7天。常压空气正常组置于笼内，未做特殊处理。采用Y型电迷宫检测大鼠学习记忆能力，HE染色观察海马锥体细胞的变化。结果高压氧治疗组模型鼠治疗后学习记忆能力提高，表现为Y型电迷宫检测尝试次数减少（30．69±0．63vs31．54±0．88，t=5．500，P=0．001），再现次数增加（3．38±0．65VS2．85±0．69，t=-2．214，P=0．047）；常压高氧治疗组治疗后大鼠学习记忆能力提高，表现为Y型电迷宫检测尝试次数减少（31．15±0．99VS31．54±0．97，t=2．739，P=0．018），再现次数增加（3．00±0．58VS2．69±0．48，t=-2．309，P=0．040）。高压氧干预后海马锥体细胞凋亡减少，正常形态细胞数增多。结论高压氧干预可提高孤独症模型鼠的学习记忆能力；高压氧干预后锥体细胞增殖，凋亡减少，可能是高压氧治疗孤独症发挥作用的途径之一。Objective To investigate the effects of hyperbaric oxygen on iearning and memory ability in rats with a model of VPA autism and on the pyramidal cells in the CA1 region of the hippocampus. Methods An animal model of autism was established in the offspring of Wistar rats which had received a single intraperitoneal injection of sodium valproate at the 12.5th day of pregnancy. A total of 48 male VPA autism model rats were randomly divided into the hyperbaric oxygen group, a high pressure air group, a normal pressure high oxygen group and a normal pressure air group （each group with 12 rats）. A normal control group was obtained by injecting physiological saline. The autism model rats of the hyperbaric oxygen group were treated with high pressure oxygen in an animal experiment cabin which was cleaned with pure oxygen for 10 min, pressurized for 15 min, held at 2.0 atmospheres absolute （ATA） for 45 min and then had the pressure relieved over 15 min. For the high pressure air group the cabin was pressurized with air for 15 min, held at 2.0 ATA for 45 rain and the pressure was relieved over 15 rain. For the normal pressure, high oxygen group the cabin was cleaned for 10 min with pure oxygen, then pure oxygen was supplied for 1 hour with the cabin door open. The normal pressure air group rats were placed in the open cabin with no pressure or additional oxygen. The testing lasted 7 days for 1 hour per day. The normal control group rats were placed in ordinary cages. Learning and memory were evaluated using the Y electric maze test before and after the interventions. Any changes in the pyramidal cells in the CA1 region of the hippocampus were observed after hematoxylin and eosin （HE） staining. Results The average number of tries of the hyperbaric oxygen group after treatment was significantly less than before treatment and memory retention times were increased. The number was also less in the normobaric hyperoxia group and memory retention times again improved. The number of apoptotic cells was reduced and thenu

关 键 词：高压氧 孤独性障碍 海马 锥体细胞 学习记忆 

分 类 号：R285.5[医药卫生—中药学]