补肾方骨青颗粒对破骨细胞分化及骨吸收功能的影响  

Effects of Guqing granule medicated serum on RANKL-induced osteoclastogenesis and bone resportion in RAW264.7 cells

作　　者：甘可[1] 冯小可[2] 郭婕[2] 谈文峰 张前德[4] 

机构地区：[1]南京中医药大学,江苏省210029 [2]南京医科大学 [3]南京医科大学第一附属医院风湿病科 [4]南京医科大学中西医结合研究所

出　　处：《江苏医药》2013年第12期1377-1380,共4页Jiangsu Medical Journal

基　　金：国家自然科学基金(30701129);教育部高等学校博士学科点专项科研基金(20093237110005)

摘　　要：目的探讨补肾方骨青颗粒对破骨细胞分化及骨吸收功能的影响。方法体外诱导破骨前体细胞系RAW264.7向破骨细胞分化,通过抗酒石酸酸性磷酸酶(TRAP)染色观察破骨细胞数目,图像分析计算骨吸收陷窝面积,荧光定量RT-PCR检测骨青颗粒对破骨细胞骨吸收功能标志性基因水平。结果与对照组相比,骨青颗粒可显著抑制TRAP染色阳性细胞的产生,减少破骨细胞骨吸收陷窝的面积,明显降低破骨细胞骨吸收功能标志性基因[组织蛋白酶K(Ctsk)、基质金属蛋白酶9(MMP-9)]mRNA的表达。结论骨青颗粒可显著抑制破骨细胞的分化及骨吸收功能。Objective To study the effects of Guqing granule medicated serum on osteoclastogenesis and bone resportion in osteoclast precursors RAW264. 7 cell line. Methods Osteoclast differentiation was induced by the receptor activator for nuclear factor xB ligand（RANKL） in RAW264. 7 cells in the presence or absence of Guqing granule medicated serum. Osteoclast formation was evaluated by tartrate-resistant acid phosphatase（TRAP） staining. Gene expressions of oste0clastic bone resportion marker was analyzed by quantitative RT-PCR. Activity of bone resorption was tested by pit formation assay. Results Guqing granule medicated serum strongly inhibited osteoclast differentiation. RANKL-induced expressions of matrix metalloproteinase-9 and cathepsin K were downregulated by Guqing granule medicated serum in the RAW264. 7 cells. Conclusion Guqing granule medicated serum is a potent inhibitor of osteoclastogenesis.

关 键 词：骨青颗粒 破骨细胞 骨吸收 

分 类 号：R285[医药卫生—中药学]