CD11c免疫脂质体的制备及其体外树突状细胞靶向性  

Preparation of CD11c modified immunoliposome and validation of its targeting to dendritic cells

作　　者：万展[1,2] 周剑[1,2] 殷萌[2] 马志强[2] 韩美娜[2] 杨峰[2] 

机构地区：[1]河北北方学院基础医学院,张家口075000 [2]第二军医大学药学院无机化学教研室,上海200433

出　　处：《第二军医大学学报》2013年第6期628-632,共5页Academic Journal of Second Military Medical University

基　　金：上海高校选拔培养优秀青年教师科研专项基金(egd09014);第二军医大学专项基金(12Js07)~~

摘　　要：目的制备CD11c单抗修饰的免疫脂质体并进行表征,验证其体外对树突状细胞(dendritic cells,DCs)的特异靶向性。方法采用薄膜分散法制备脂质体,通过化学交联法将CD11c单抗连接于脂质体上,利用高效液相法测定CD11c与脂质体的连接率,透射电镜观察脂质体形态,激光粒度测定仪测定粒径和Zeta电势,流式细胞仪检测脂质体上连接CD11c的活性和对DCs的靶向效果,激光共聚焦显微镜观察该免疫脂质体特异性靶向特点。结果成功制备了CD11c免疫脂质体,测得单抗连接率为(77.6±3.2)%,平均粒径为(151.2±1.6)nm,Zeta电位为(-32.0±1.8)mV。电镜下脂质体粒径均匀,表面光滑,多为圆形,少许椭圆形,无粘连。CD11c单抗连接于脂质体仍保持了良好的活性,体外表现出显著的DCs特异靶向性。结论制备的CD11c免疫脂质体对DCs细胞具有特异靶向性。Objective To prepare and characterize liposorne with mAb（CDllc-imliposomes） and to validate its targeting to dendritic cells （DCs） in vitro. Methods Liposornes were prepared by film dispersion method, and CDllc mAb was collected to the surface of liposome by chemical cross linking method. The conjugation efficiency of CDllc mAb to the liposorne was determined by H PLC; the morphology of CDllc-irnliposomes was observed under transmission electron microscope; the particle size and Zata potential were determined by dynamic light scattering; the activity of CDllc mAb was examined by flowcytometry; and its specific targeting to DCs was visualized by laser scanning confocal microscopy. Results The CDI lc-lmnposomes were successfully prepared and the conjugation efficiency was （77.6±3.2）%, with the particle size being （151. 2±1.6） nm and the Zeta potential being （-32.0±1.8） inV. Under microscope, the CDllc-imliposomes were mostly round in shape or elliptical, with smooth surface and with no adhesion. The activity of CDllc-imliposomes was still satisfactory and demonstrated great targeting to DCs in vitro. Conclusion The CDllc-imliposomes prepared by us has targeting ability to DCs in vitro.

关 键 词：CD11c抗原 脂质体 树突状细胞 靶向 

分 类 号：R943.42[医药卫生—药剂学]