急性低温暴露对家兔心肝肾功能的影响  

Effects of Acute Cold Exposure on Rabbit Heart, Liver and Renal Function

作　　者：吴丽珍[1,2] 杜兴华[3,2] 雷艳[2,4] 蔡颖[2] 董兆君[2] 

机构地区：[1]云南省中西医结合医院功能科,云南昆明650224 [2]第三军医大学军事预防医学院毒理学研究所 [3]云南省中西医结合医院检验科,云南昆明650224 [4]第三军医大学基础部外科应用解剖与手术学教研室,重庆400038

出　　处：《昆明医科大学学报》2013年第5期43-49,共7页Journal of Kunming Medical University

基　　金：军队"十一五"医学科研攻关基金资助项目(06G077)

摘　　要：目的探讨急性低温暴露对家兔心、肝、肾功能的影响.方法 15只家兔随机分为3组,20℃组、-20℃组、2℃组,每组5只.动物经腹腔注射麻醉后行左侧颈总动脉插管术,按分组分别置于20℃室温、2℃和-20℃实验冰柜中.各组在置于实验温度后0、30、60、90、120 min时间点经颈动脉插管采血并分离血浆,采用生化方法测定肝功能指标包括门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、碱性磷酸酶(ALP)、L-γ谷氨酰基转移酶(GGT);肾功能指标尿素(BU)、肌酐(CREA)、尿酸(UA);心肌酶谱指标包括门冬氨酸氨基转移酶(AST)、肌酸激酶(CK)及同工酶(CKMB)、乳酸脱氢酶(LDH)和α一羟丁酸脱氢酶(a-HBDH)的含量.结果急性低温暴露能显著影响家兔心、肝、肾功能血生化指标,-20℃暴露组更明显.结论急性低温暴露可对家兔心、肝、肾功能造成损伤,低温暴露强度越强损伤越明显.Objective To explore the effects of acute cold exposure on the rabbits heart, liver and renal function. Methods 15 rabbits were randomly divided into 3 groups, 20% group, -20℃ group and 2% group, with 5 rabbits in each group. Animals were anesthetized by intraperitoneal injection, then were treated with the left common carotid artery intubation. According to the grouping, they were exposed to freezer at 20%, 2℃ and -20℃, respectively. At 0, 30, 60, 90 and 120 rain after exposure, the blood was collected by the way of carotid intubation, and plasma was separated. Then, the biochemical method was used to determine the contents of liver function indexes including aspartate aminotransferase （AST） , alanine aminotransferase （ALT） , alkaline phosphatase （ALP） and L- γ-glutamyl transferase （GGT） ; of renal function indexes including urea （BU） , creatinine （CREA） , uric acid （UA） ; and of heart function indexes including aspartate aminotransferase myocardial enzyme （AST） , creatine kinase （CK） and isoenzyme （CKMB） , lactate dehydrogenase （LDH） and α-hydroxybutyrate dehydrogenase （ α-HBDH） content. Results Acute exposure to low temperature could significantly affect the heart, liver and renal function indexes in rabbits, and exposed in -20 ℃ was the most obvious. Conclusion Acute cold exposure can cause damage to the heart, liver and renal function in rabbits, and the lower temperature has the more obvious damage.

关 键 词：急性低温暴露 心肝肾功能 血生化指标 损伤 

分 类 号：Q494[生物学—生理学]