直接酸化原子吸收光谱法测定血清中微量元素铬和锰  

Determination of trace elements chromium and manganese in serum of direct acidification by atomic absorption spectrometric

作　　者：马振菁 李新民[2] 陈发林[3] 阙慧卿 钱丽萍 林绥 

机构地区：[1]福建省医学科学研究院医学测试重点实验室,福州350001 [2]福建省立医院消化内科 [3]福建省立医院检验科

出　　处：《福建医药杂志》2013年第3期63-65,共3页Fujian Medical Journal

基　　金：福建省自然科学基金项目(2010J01134)

摘　　要：目的建立原子吸收光谱法测定血清中微量元素铬(Cr)和锰(Mn)的含量。方法采用直接硝酸酸化处理血清蛋白,优化石墨炉的灰化原子化温度。用有证国家一级标准物质GBW(E)090006评价正确度。结果本方法线性范围为0.50～10.00μg/L;Cr、Mn的加标回收率分别为98.50%～100.25%、95.80%～103.40%;相对标准偏差分别为0.48%～1.32%、0.89%～2.06%;最低检测限分别为0.13μg/L、0.03μg/L。结论本方法简便、精密度好,检出限低,准确度高,可推荐作为临床血清铬和锰测定的参考方法。Objective To establish atomic absorption spectrometric method to determine the content of trace elements chromium （Cr） and manganese （Mn）. Methods We used nitric acid to acidify serum protein directly, optimized ashing and atomic temperature of graphite furnace, and evaluated the accuracy rating by national primary standard substance GBW （E） 090006 with a certificate. Results The linear range of the method was 0. 50-10. 00υg/L; standard added recovery of Cr and Mn were 98.50%-100.25%and 95.80%-103.40%, respectively; the relative standard deviation of Cr and Mn were 0.48%-1.32% and 0.89%-2.06%, respectively ; the minimum detection limit of Cr and Mn were 0.13 υg/L and 0.03 υg/L, respectively. Conclusion This method is simple furthermore, its forte includes good precision, low detection limit, and high accuracy. It can be recommended as a reference method for clinical determination of chromium and manganese in serum.

关 键 词：原子吸收光谱法 铬 锰 血清 微量元素 

分 类 号：R446.1[医药卫生—诊断学]