ELFMFs诱导大鼠腓肠肌钝挫伤恢复的组织学、超微结构及血清酶活性的影响  

Changes of ultrastructural and serum enzyme activity in rats gastrocnemius blunt injury recovery induced by ELFMFs

作　　者：董贵俊[1] 王超[1] 葛新发[1] 潘卫东[2] 李可峰[1] 

机构地区：[1]山东体育学院,山东济南250102 [2]中国科学院电工研究所生物医学工程部,北京100190

出　　处：《山东体育学院学报》2013年第3期37-40,共4页Journal of Shandong Sport University

摘　　要：极低频电磁场能加快组织愈合、促进细胞分化等,但是对骨骼肌钝挫伤后恢复过程中的作用研究甚少。用极低频电磁场对骨骼肌钝挫伤大鼠模型进行处理,并通过组织学观察及生理、生化相关指标检测来分析判断ELFMF对骨骼肌损伤恢复的影响。结果发现,与假暴露组相比,磁场暴露组肌细胞排列更整齐,炎性细胞浸润少,细胞水肿程度轻,核内移现象少;磁场暴露组、注射bFGF组肌纤维排列紊乱程度、Z线飘移程度均较轻;极低频电磁场处理,能显著降低钝挫伤后血清CK、LDH及ALP水平。因此,极低频电磁场通过改变钝挫伤后腓肠肌肌细胞核增生,加快线粒体自身修复速度,减少肌细胞膜的通透性,加速受损肌细胞有氧代谢能力的恢复。极低频电磁场通过影响肌细胞核及线粒体的增生,缓解受损骨骼肌的能量危机,从而加速受损肌组织恢复速度。Magnetic field therapy is suggested to enhance healing of injuries and promote cell differentiation, but lack of researches on skeletal muscle contusion in the restoration process of action. In this study we used extremely low frequency magnetic fields （ELFMFs） on skeletal muscle contusion in rat model, and by histological observation and physiological, biochemical indexes related to detection to analyze the ELFMF effects on skeletal muscle injury recovery. Compared with the natural control animals, the microcytes of sham animals showed no obvious mitochondria structure as well as myofibrils blurred and Z -line aligned disorderly. Length differences of sarcomere among the bFGF group, the exposed group and the sham group were significant （P 〈 0.05 ）. In terms of histochemistry, the exposed group and the injection group showed better recovery than the sham group. In terms of biochemistry, ferritin and transferrin in serum or tissue of individual group were determined and analyzed. The detailed mechanisms involved were still under investigation. ELFMFs could change contusion gastrocnemius muscle cell nuclei hyperplasia, accelerate mitochondrial repair speed, reduce muscle cell membrane permeability and accelerate the recovery rate of aerobic capacity in muscle cells. Therefore,ELFMFs can influence the regeneration of nuclei and mitochondria in iinpaired skeletal muscle, alleviate the energy crisis,inorder to accelerated the damaged muscle tissue recovery rate.

关 键 词：极低频电磁场 腓肠肌 超微结构 血清酶 钝挫伤 

分 类 号：G804.7[文化科学—运动人体科学]