CNTF对烧伤大鼠血清引起大鼠海马神经元细胞毒性的影响  

CNTF PREVENTS SERUM OF BURN RAT MEDIATED CYTOTOXICITY IN CULTURED HIPPOCAMPAL NEURONS

作　　者：陈秀青[1] 黄爱军[1] 路长林[1] 王成海[1] 鲍璿[2] 

机构地区：[1]第二军医大学神经科学研究所,神经生物学教研室,上海200433 [2]中国科学院上海脑研究所,上海200031

出　　处：《细胞生物学杂志》2000年第1期43-47,共5页Chinese Journal of Cell Biology

摘　　要：应用整体和离体神经元培养,观察CNTF对烧伤大鼠海马神经元及烧伤血清引起海马神经元损伤的影响,结果表明,大鼠烧伤后海马组织神经元数目减少,NO含量升高;烧伤大鼠血清可引起培养的海马神经元细胞存活率下降,培养液中NO含量升高;CNTF能降低烧伤大鼠海马组织中NO的含量,保护海马神经元,并能提高培养的海马神经元的存活率,减少培养液中NO含量,其作用呈剂量依赖性;CNTF对神经元存活率的影响与NO含量呈显著负相关,提示CNTF对烧伤大鼠血清引起的海马神经元损伤有保护作用,其作用机制可能是通过抑制NO的神经毒性。Using primary cultures of rat hippocampal neurons and the model of burn rats,observed the effects of ciliary neu-rotrophic factor on contents of NO and the number of neurons on hippocampus,observed effects of CNTF on the neurons survival and the content of NO which induced by serum of burn rat. The result showed that the numbers of hippocampal neuron decreased and the contents of NO increased in burn rats. The serum of burn rats could induce the cell survival rate decreased andthe levels of NO increased. Ciliary neurotrophic factor (CNTF) could promoted neurons survived,decreased NO level which induced by serum of burn rat. The protective effects of CNTF against cytotoxicity were dependent on concentrations. Conclusion:These findings suggested that CNTF protected hippocampal neurons a-gainst serum of burn rat induced injury by preventing NO neurototoxicity.

关 键 词：CNTF 烧伤 海马神经元 NO 神经毒性 保护作用 

分 类 号：R644[医药卫生—外科学]