新生儿缺氧缺血性脑病患儿外周血CD4^+ CD25^+调节性T细胞的变化及其意义  

The Changes and Significance of CD4^+ CD25^+ Regulatory T Cell in Peripheral Blood of Neonates with Hypoxic-ischemic Encephalopathy

作　　者：孙林[1] 宋继成[1] 周旭华[1] 

机构地区：[1]连云港市妇幼保健院,江苏连云港222006

出　　处：《标记免疫分析与临床》2013年第3期135-137,共3页Labeled Immunoassays and Clinical Medicine

摘　　要：目的探讨CD4+CD25+调节性T细胞在新生儿缺氧缺血性脑病(HIE)患儿外周血的改变,及其在HIE疾病进程中的意义。方法用流式细胞术分析HIE组患儿92例和对照组31名外周血CD4+CD25+T细胞的变化,及其细胞内foxp3的表达变化。结果轻度、中度和重度HIE组患儿外周血CD4+CD25+T细胞比例分别为(9.26±1.18)%、(6.51±1.35)%和(5.36±1.54)%,对照组为(8.42±2.02)%。轻度HIE组患儿外周血CD4+CD25+T细胞较对照组有增高趋势,但差异无统计学意义(P>0.05);中度和重度HIE组患儿CD4+CD25+T细胞明显低于对照组,差异有统计学意义(P<0.05)。轻度、中度和重度HIE组患儿外周血CD4+CD25+foxp3+T细胞分别为(1.36±1.14)%、(1.21±0.94)%和(1.09±0.54)%,对照组为(1.30±1.17)%。轻度HIE组患儿CD4+CD25+foxp3+T细胞较对照组有增高趋势,但差异无统计学意义(P>0.05)。中度和重度HIE组患儿CD4+CD25+foxp3+T细胞明显低于对照组,差异有统计学意义(P<0.05)。结论中度和重度HIE组患儿CD4+CD25+foxp3+T细胞明显减少,这些调节性T细胞可能参与HIE的病理进程。Objective To explore the changes of CD4＋CD25＋ regulatory T cells in peripheral blood of neonatal patients with hypoxic-ischemic encephalopathy（HIE）,and its significance in the process of HIE.Methods The levels of CD4＋CD25＋Tcells and changes of the foxp3 expression in 92 patients with HIE and 31 health children were detected by flow cytometry.Results The levels of CD4＋ CD25 ＋ T cells in the peripheral blood of moderate and severe HIE neonates were significantly lower than that of controls（P0.05）.The foxp3 expression in CD4＋CD25＋ T cells in peripheral blood of moderate and severe HIE neonatal were significantly lower than that the controls（P0.05）.Conclusion The CD4＋CD25＋ foxp3＋ T cell in moderate and severe HIE children are significantly reduced,and these regulatory T cells may be involved in the pathological process of HIE.

关 键 词：新生儿缺氧缺血性脑病 外周血 CD4+CD25+T细胞 CD4+CD25+foxp3+T细胞 流式细胞术 

分 类 号：R722.1[医药卫生—儿科]