角形自由流电泳装置的设计与应用研究  

Fabrication and Examination of a Novel Triangular-shape Free Flow Electrophoresis Device

作　　者：张衡瑞[1,2] 李建波 于好兴 董靖宇[2] 曹成喜[4] 张维冰[1,2] 

机构地区：[1]齐齐哈尔大学,齐齐哈尔161006 [2]华东理工大学,上海200237 [3]山东中矿集团有限公司,招远265401 [4]上海交通大学,上海200240

出　　处：《分析化学》2013年第6期856-860,共5页Chinese Journal of Analytical Chemistry

基　　金：国家重大科学仪器研制专项基金(No.2011YQ030139);国家自然科学基金(No.210750390);中央高校基本科研业务费专项资金(No.WK1014042)赞助

摘　　要：基于自由流电泳(Free flow electrophoresis,FFE)原理设计一种新型角形FFE装置,与传统矩形装置相比,新型装置分离腔设计为角形,运行时单管进样,样品由泵推动前移形成层流,避免缓冲液使用,显著降低了仪器运行成本。研究了角形装置构造和电场分布理论,在优化的分离条件U=400 V、v=0.5 mL/min时,成功分离了中性红和亚甲基兰混合液。在传统矩形装置上U=70 V、v=1.0 mL/min条件下,山东某金矿贵液经分离,杂质铜与锌降低至51.46%与58.87%;相同条件下,在角型FFE装置上,铜与锌减少至52.77%和53.94%,即角形装置可以达到与传统矩形装置同等分离效果,并减少了背景缓冲液的使用。因此,此装置有望在大规模样品连续分离方面发挥重要作用。A novel triangular-shape FFE device was designed based on free flow electrophoresis（FFE） theory.In comparison with traditional FFE device,the shape of separation chamber of the novel device was triangular.The sample was driven forward by pump to form laminar flow when applied single-tube sample injection without the background buffer.The structure of the device was studied as well as the electric field distribution theory.The mixture of methylene blue and neutral red was successfully separated on this device when voltage was 400 V and flow rate was 0.5 mL/min.Traditional rectangle-shape device could reduce the copper and zinc contents of Shangdong noble metallic solution to 51.46% and 58.87%,respectively,when U=70 V and v=1.0 mL/min.The contents of copper and zinc could be reduced to 52.77% and 53.94% after the disposal of triangular-shape device when U=70 V,v=1.0 mL/min and third and fourth electrode chambers were in series with 500 Ω and 1000 Ω,respectively.Thus,the triangular-shape device could reach the similar separation effect with traditional rectangle-shape device.Therefore,this device might be promising in the field of consecutive separation of macroscale samples.

关 键 词：自由流电泳 场强分布 矩形装置 角形装置 金矿贵液 分离 

分 类 号：Q51-33[生物学—生物化学]