基于CSSL的水稻苗期耐亚铁离子胁迫QTL定位  

Mapping of quantitative trait loci associated with ferrous iron toxicity tolerance at seedling stage based on chromosome segment substitution line in rice

作　　者：赵春芳[1] 周丽慧[1] 丁丹[1] 张亚东[1] 赵庆勇[1] 于新[1] 朱镇[1] 陈涛[1] 姚姝[1] 王才林[1] 

机构地区：[1]江苏省农业科学院粮食作物研究所,江苏省优质水稻工程技术研究中心,国家水稻改良中心南京分中心,江苏南京210014

出　　处：《江苏农业学报》2013年第3期461-467,共7页Jiangsu Journal of Agricultural Sciences

基　　金：现代农业产业技术体系建设专项资金项目(CARS-01-47);江苏省农业科技自主创新基金项目[CX(12)1003]

摘　　要：以籼稻品种9311为遗传背景携带日本晴置换片段的119个染色体片段置换系(CSSL)为试验材料,以相对根长为耐性指标,利用代换作图方法对水稻苗期耐Fe2+胁迫QTL进行了定位。结果表明,以P≤0.01为阈值,筛选到相对根长与受体亲本9311差异极显著的14个CSSL,共鉴定了9个耐Fe2+胁迫QTL,分布于水稻第2、5、6、7和12染色体上,其中4个QTL在含1个重叠片段的CSSL中被检测到,其余5个QTL在含2～3个重叠片段的CSSL中被检测到。9个耐Fe2+胁迫QTL中,qRRL12的加性效应值为负,效应来源于对Fe2+胁迫敏感的亲本9311,其余8个QTL的加性效应值为正,其加性效应值变化范围为7.63～19.88,加性效应百分率的变化范围为13.69%～35.66%,效应均来源于耐Fe2+胁迫的亲本日本晴。One hundred and nineteen chromosome segment substitution lines （CSSLs） developed from two se- quenced rice cuhivars, the indica cultivar 9311 as recipient, and the japonica cultivar Nippobare as donor, were used to detect QTL for relative root length under Fe2＋ stress. After compared to the recurrent parent 9311 by t test, 14 CSSLs with significance were selected at P＋〈0.01 level. Using substitution mapping strategy, nine QTL were detected on rice chromo- somes 2. 5. 6. 7 and 12. of which four were identified one substituted sezment of CSSL. while the others were identified from more than two substituted segments of CSSL. Among the nine QTL, qRRL12 had a negative additional effect derived from the Fe2＋ sensitive parent 9311. Other 8 QTL had a positive additional effect all derived from the Fe2＋ tolerant parent Nippobare. The additive effects of these QTL ranged from 7. 63 to 19. 88 and additive effect from 13.69% to 35.66%.

关 键 词：水稻 亚铁离子胁迫 根长 染色体片段置换 QTL 

分 类 号：S511.032[农业科学—作物学]