棉花草甘膦抗性基因CP4-EPSPS的初步定位  

Mapping of glyphosate-resistant gene CP4-EPSPSin cotton

作　　者：刘吉焘[1] 马晓杰[2] 狄佳春[2] 陈旭升[2] 

机构地区：[1]南京农业大学农学院,江苏南京210095 [2]江苏省农业科学院经济作物研究所/农业部长江下游棉花与油菜重点实验室,江苏南京210014

出　　处：《江苏农业学报》2013年第3期480-484,共5页Jiangsu Journal of Agricultural Sciences

基　　金：转基因生物新品种培育科技重大专项子课题(2011ZX08005-001)

摘　　要：以抗草甘膦陆地棉品系G-6和不抗草甘膦海岛棉品系海7124为试验材料,对分离世代进行检测,分析抗性基因的遗传规律;利用覆盖棉花26条染色体的234对核心引物,通过群体分离分析法进行差异性标记筛选,利用F2分离群体对抗性基因进行染色体定位。结果表明,抗草甘膦性状是受1对显性基因控制的质量性状。特异引物检测显示控制抗草甘膦性状的基因为人工合成基因CP4-EPSPS。利用筛选获得的27对多态性引物检测F2作图群体每个单株的基因型,发现分子标记NAU5417、NAU1339、BNL3992、BNL2448、NAU2140与目的基因CP4-EPSPS连锁。进一步筛选,共得到15个分子标记。参照现有的遗传图谱,推断目的基因CP4-EPSPS位于棉花第5染色体BNL2448与NAU2140之间,遗传距离分别为7.0 cM和16.2 cM。G-6 （glyphosate-resistant cotton） and Hai 7124 （non-resistant sea island cotton） were used in this study. The hybrid F1 population was resistant to glyphosate. The ratio of resistant plants to non-resistant ones in F2 population was consistent with 3 ： 1, and the ratio in recessive baekcross population BC2 was consistent with 1 ： 1, indicating that glyphosate-resistant trait was controlled by a pair of dominant genes. The glyphosate-resistant gene was detected to be an artificially synthesized one, CP4-EPSPS, using a pair of distinctive primers. By bulk segregation analysis, a total of 27 polymorphic primers were obtained from 234 pairs covering 26 pairs of chromosomes in cotton, among which 5 pairs of primers, NAU5417, NAU1339, BNL3992, BNL2448 and NAU2140 were found to be linked with target gene CP4-EPSPS. Further screening showed 15 pairs of primers linked to the gene. According to a known genetic map, CP4-EPSPS was located on BNL2448 and NAU2140 of the fifth chromosome, with genetic distances of 7.0 cM and 16.2 cM ,respectively.

关 键 词：棉花 抗草甘膦 CP4-EPSPS SSR标记 基因定位 

分 类 号：S562.034[农业科学—作物学]