猪链球菌2型体外刺激3D4/21猪肺泡巨噬细胞的炎症相关细胞因子mRNA转录分析  

mRNA level of inflammation-associated cytokines of porcine alveolar macrophages cell lines 3D4/21 stimulated by Streptococcus suis serotype 2

作　　者：汪伟[1] 何孔旺[1] 倪艳秀[1] 吕立新[1] 周俊明[1] 张雪寒[1] 俞正玉[1] 茅爱华[1] 温立斌[1] 王小敏[1] 李彬[1] 郭容利[1] 

机构地区：[1]江苏省农业科学院兽医研究所,农业部兽用生物制品工程技术重点实验室,国家兽用生物制品工程技术研究中心,江苏南京210014

出　　处：《江苏农业学报》2013年第3期559-564,共6页Jiangsu Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(31072155);江苏省自然科学基金重点项目(BK2010068);江苏省农业科技自主创新基金项目[CX(11)2060]

摘　　要：为探明猪链球菌2型(SS2)感染的致病机制,利用荧光定量PCR方法,分析不同SS2菌株对3D4/21猪肺泡巨噬细胞各炎症相关细胞因子mRNA体外转录水平的影响。结果显示强毒菌株ZY05719刺激细胞因子转录水平高于其impdh缺失株ZY05719(Δimpdh)和无毒菌株HA0609及低毒菌株CS100322-1。ZY05719诱导多个细胞因子mRNA过度转录,包括IL-18、IL-8和IL-12等。除IL-10外,HA0609诱导其他细胞因子mRNA上调转录;而CS100322-1刺激后,绝大部分细胞因子mRNA处于稳定状态。不同毒力SS2菌株影响细胞因子mRNA转录,且这种影响具有时间上的依赖性。SS2具有调节炎性细胞因子转录的能力,其结果介导炎症反应及白细胞趋集于感染部位,这可能是SS2引起脑膜炎炎症特征的机制之一。To study the pathogenesis of Streptococcus suis serotype 2 （SS2） infection, fluorogenic quantitative PCR was used to detect mRNA transcriptional level of inflammation-associated cytokines of porcine alveolar macrophages 3134/21 stimulated by SS2. The transcriptional level of cytokine, induced by high virulent strain ZY05719, was higher than that induced by impdh-deficient mutant ZY05719 （Aimpdh）, HA0609 （avirulent SS2 strain） and CS100322-1 （low virulent SS2 strain）. When IL-18, IL-8 and IL-12 were over-transcripted when induced by ZY05719. All of tested cytokines were upregulated when induced by HA0609, except IL-10. However, the cytokines induced by CS100322-1 stayed stable. Different virulent strains of SS2 seemed to influence the mRNA transcriptional level of cytokines, which was time-dependent. SS2 was capable of regulating important inflammation-associated cytokines resulting in mediating inflammatory reaction and an increased leucocyte recruitment into the sites of infection, which might provide a possible explanation for some of inflammatory features of meningitis caused by SS2.

关 键 词：猪链球菌2型 猪肺泡巨噬细胞 炎症相关细胞因子 荧光定量PCR 

分 类 号：S858.282.61[农业科学—临床兽医学]