丙型肝炎病毒核酸定量和抗-HCV血清学分析的诊断价值  

Retrospective analysis of Antibody of HCV and quantification of HCV RNA in diagnosis of hepatitis C

作　　者：刘贤华[1] 白晓东[2] 刘元明[3] 段萃娟[3] 荣冉[3] 刘维维[2] 

机构地区：[1]武警总医院医疗科,北京100039 [2]武警总医院烧伤整形科,北京100039 [3]武警总医院检验科,北京100039

出　　处：《武警医学》2013年第6期488-490,共3页Medical Journal of the Chinese People's Armed Police Force

摘　　要：目的探讨PCR-荧光探针法与抗-HCV检测在丙型肝炎(丙肝)诊断中的应用价值。方法回顾性分析265例疑似丙肝病毒感染者行HCV-RNA、抗-HCV及ALT检测的资料,统计不同年龄、性别分布情况,进行HCV-RNA与抗-HCV、ALT的相关性分析。结果 (1)男性抗-HCV与HCV-RNA的阳性率均高于女性,但差异无统计学意义(P>0.05);年龄>40岁的患者抗-HCV与HCV-RNA的阳性率明显高于年龄≤40岁的患者,差异有统计学意义(P<0.05)。(2)ALT异常率随HCV-RNA阳性率的增高而增加,并与HCV-RNA含量成正相关(r=0.999,P<0.05)。但ALT的水平与HCV-RNA含量无相关性(r=0.800,P>0.05)。(3)HCV-RNA阳性多伴有抗-HCV阳性(r=0.320),二者有很好的相关性。结论 ELISA法检测抗-HCV在丙型肝炎诊断中仍旧为首选检测方法。联合应用HCV-RNA和抗-HCV及ALT检测,是诊断丙型肝炎最为可靠的方法。Objective To study the application value of hepatitis C virus RNA detection（PCR-fluorescent probe） and hepatitis C virus antibody detection（ELISA,anti-HCV） in the diagnosis of hepatitis C.Methods The HCV-RNA and anti-HCV tests of 265 suspected HCV patients were reviewed,statistics of anti-HCV and HCV-RNA detection results and ALT level in different ages and gender was collected and analysized.Results ①Anti-HCV and HCV-RNA positive rates in men were higher than those in women,but there was no statistically significant difference（P〈0.05）.② Anti-HCV and HCV-RNA positive rates in 40 years old patients were obviously higher than those in ≤40 years old patients,with statistically significant difference（P〈0.05）;ALT abnormal rates also had significant difference between 40 and ≤40 years old patients（P〈0.05）.③ ALT abnormal rate increased with increasing HCV-RNA positive rate and have positive correlation with HCV-RNA content（r = 0.999,P〈0.05）.But the ALT levels did not correlate with HCV-RNA content（r = 0.800,P〈0.05）.④The abnormal rate of Anti-HCV was closely correlated with HCV-RNA（r = 0.320）;Anti-HCV,HCV-RNA and ALT had no statistically significant difference（P〈0.05）.Conclusions ①Anti-HCV and HCV-RNA detection have no relation with gender,but they have certain relevance with age.②ELISA for detecting anti-HCV is still first choice in HCV.Combined use of HCV-RNA,anti-HCV and ALT detection,will be the most reliable and valuable method in diagnosis of HCV.

关 键 词：丙型肝炎病毒核酸定量检测 抗-HCV 诊断 

分 类 号：R512.63[医药卫生—内科学]