蛇毒抗菌的比较性研究  

Comparative analyses of the bactericidal activity of snake venoms

作　　者：李妲[1] 孙明忠[2] 郭春梅[2] 彭博雅[2] 刘淑清[1] 

机构地区：[1]大连医科大学生化教研室,辽宁大连116044 [2]大连医科大学生物技术系,辽宁大连116044

出　　处：《中国微生态学杂志》2013年第6期639-642,共4页Chinese Journal of Microecology

基　　金：辽宁省高等学校杰出青年学者成长计划项目(LJQ2011094);辽宁省"百千万人才工程"资助项目(2012921015);国家自然科学基金(81100722)

摘　　要：目的研究蛇毒粗毒的抗菌作用,并比较不同蛇毒对不同细菌的抑制效果。方法采用抑菌环法测定蛇岛蝮、江浙蝮和短尾蝮蛇毒粗毒对金黄色葡萄球菌、枯草芽孢杆菌、大肠埃希菌、铜绿假单胞菌以及白色念珠菌的抗菌作用,比较不同蛇毒粗毒的抗菌效果;加入过氧化氢酶(catalase),考察蛇毒抗菌作用的变化。结果 (1)3种蛇毒粗毒对5种细菌均呈现不同程度的抑制作用。(2)3种蛇毒粗毒对5种细菌的抑制作用大小依次为:金黄色葡萄球菌>白色念珠菌>大肠埃希菌或铜绿假单胞菌>枯草芽孢杆菌。(3)加入catalase后,蛇毒的抗菌作用明显减弱。结论 (1)3种蛇毒粗毒均具有一定的抗菌作用,并具有明显抑菌选择性。(2)3种蛇毒的抗菌效果不同,蛇岛蝮蛇抗菌作用最强。(3)catalase能显著降低蛇毒的抗菌活性,表明L-氨基酸氧化酶是蛇毒起抗菌作用的主要成分。Objective To measure and compare the anti-bacterial activity of crude snake venoms against different bacterium strains.Methods The bacteria-inhibiting ring method was applied to measure the anti-bacterial effects of the snake venoms of Gloydius shedaoensis,Agkistrodon halys Pallas and Gloydius brevicaudus on the growths of the bacterium strains of Staphyloccocus aureus,Bacillus subtilis,Escherichia coli,Pseudomonas aeruginosa and Candida albicans.The anti-bacterial activities of different crude snake venoms were then compared.The effect of the addition of catalase was also compared.Results（1） The three crude snake venoms showed varied inhibitory effects on the five types of bacterium tested in current work.（2） Basically,the snake venoms showed the strongest inhibitory effect against Staphyloccocus aureus,followed by Candida albicans,Escherichia coli or Pseudomonas aeruginosa and Bacillus subtilis.（3） In the presence of catalase,the anti-bacterial effect of Gloydius shedaoensis vemon was significantly suppressed.Conclusions（1） The three snake venoms all showed anti-bacterial activities,with varied anti-bacterial selectivity.（2） Among them,Gloydius shedaoensis vemon showed the highest anti-bacterial activity.（3） The addition of catalase dramatically decreased the anti-bacterial activity of crude snake vemon,which indicates that L-amino acid oxidase（LAAO） is the critical component responsible for the anti-bacterial activity of snake venoms.

关 键 词：蛇毒粗毒 抗菌 L-氨基酸氧化酶 过氧化氢酶 

分 类 号：R378.99[医药卫生—病原生物学]