三株H9亚型禽流感病毒HA基因的遗传进化分析  

Phylogenetic Analysis of HA Genes of Three H9 Subtype Avian Influenza Viruses

作　　者：高如一[1] 缪晓斌[2] 宿春虎[1] 陈素娟[1] 王晓泉[1] 刘晓文[1] 彭大新[1] 

机构地区：[1]扬州大学兽医学院,江苏扬州225009 [2]江苏省东台市畜牧兽医站,江苏东台224220

出　　处：《中国家禽》2013年第12期17-20,共4页China Poultry

基　　金：江苏省大学生实践创新训练计划(2012JSSPITP1404);江苏高校优势学科建设工程项目

摘　　要：2012年初从东台地区分离到3株H9亚型禽流感病毒,通过RT-PCR扩增出其HA基因,并对其进行测序及遗传进化分析。结果显示3株病毒的HA基因开放阅读框全长1683bp,共编码560个氨基酸,其核苷酸和氨基酸的同源性分别为95.8%～97.8%、97.7%～98.9%,与A/chicken/F/98的同源性分别为90.9%～91.4%、93.2%～93.9%;其裂解位点均为PSRSSR↓GLF,符合低致病力禽流感毒株的特征,推导的氨基酸序列含8个相同的潜在糖基化位点,A/chicken/DT/1/2012比其他2株H9亚型AIV多了一个267NCT的糖基化位点;受体结合位点的226位为L,具有α-2,6受体结合的特征。遗传进化分析显示3株分离株属欧亚种系,与A/Duck/Hong Kong/Y280/97代表株的亲源关系较近。The hemagglutinin （HA）genes of three H9 subtype avian influenza viruses （AIVs）isolated in Dongtai area in 2012 were amplified by RT-PCR and their sequences were analysed. The results showed that HA genes of AIV isolates were composed of 1 683 bp nueleotides, encoding 560 amino acids. Nucleotide and amino acid homologies among these AIV isolates were from 95.8% to 97.8% and 97.7% to 98.9%,respectively. They shared 90.9% to 91.4% and 93.2% to 93.9% homologies with A/chicken/F/98 strain. The cleavage site of HA proteins was PSRSSR ↓ GLF,which had the characteristic of low pathogenic AIV strain. There were eight potential glycosylation sites in the HA genes of three isolates, and A/chicken/DT/1/2012 strain had an additional glycosylation site at position 267. The Leucine at position 226 in the HA genes of 3 isolates indicated the potential of binding with SA α,2-6 receptor. Phylogenetic analysis of HA genes showed that 3 isolates belonged to Eurasian lines,which had a close relationship with A/Duck/ Hong Kong/Y280/97 AIV strain.

关 键 词：H9 禽流感病毒 血凝素基因 遗传进化分析 

分 类 号：S852.659.5[农业科学—基础兽医学]