应用环磷酰胺研制细胞表面抗原的单克隆抗体  

The preparation of monoclonal antibody against cell membrane by cyclophosphamide

作　　者：周王景 马宝骊[1] 严俊[1] 

机构地区：[1]上海第二医科大学免疫学教研室,上海200025

出　　处：《微生物学免疫学进展》2000年第3期26-28,共3页Progress In Microbiology and Immunology

摘　　要：利用环磷酰胺和杂交瘤技术 ,成功制备了 1株稳定分泌抗人CD80 分子单克隆抗体的杂交瘤细胞。实验结果表明 ,该单抗腹水效价为 1 0 6 ,属IgG1亚类 ,特异性强 ,为今后的应用打下了基础。One stable hybridoma cell line secreting McAb anti CD 80 has been established.The McAb belongs to IgG1 subclass.The titer of McAb in ascites was 10 6 .The specificity of McAb were identified by cell ELISA and FACS.The results above have laid foundation for the application.

关 键 词：环磷酰胺 细胞表面抗原 单克隆抗体 免疫删减 

分 类 号：R392.11[医药卫生—免疫学]