不同中药复方对脑缺血再灌注大鼠凝血与纤溶相关指标影响的实验研究  

Present Research on Effects of Different Traditional Medicine on Indexes Related to Thrombin and Plasma in Testing Blood Clotting and Plasm Indicatrix of Rats with Cerebral Ischemia Reperfusion

作　　者：俞丽华[1] 王键[1] 胡建鹏[1] 陈业农[1] 王业梅[1] 张娟[1] 

机构地区：[1]安徽中医药大学,安徽合肥230038

出　　处：《辽宁中医药大学学报》2013年第7期140-142,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：安徽省自然基金项目(1208085QH150);安徽中医药大学自然基金项目(2012zr007)

摘　　要：目的:探讨不同的中药复方对大鼠脑缺血再灌注血浆凝血与纤溶相关指标血管性血友病因子(vWF)、组织型纤溶酶原激活物(t-PA)和组织型纤溶酶原激活物抑制物(PAI-1)的动态影响。方法:采用线栓法制作大鼠局灶性脑缺血再灌注模型,随机分为假手术组、模型组、益气活血方组、镇肝熄风汤组和星蒌承气汤组,分别给予相应药物灌胃;采用酶联免疫法检测血浆vWF、tPA和PAI-1含量。结果:模型组再灌注各时间点血浆vWF、t-PA含量显著升高,PAI-1血浆含量显著下降;各治疗组再灌注各时间点血浆vWF、t-PA含量显著降低和PAI-1含量显著升高;在降低vWF方面,24 h潜阳熄风组优于化痰通腑组和益气活血组,48 h化痰通腑组优于潜阳熄风组和益气活血组,72 h益气活血组优于潜阳熄风组和化痰通腑组;在降低血浆t-PA,24 h化痰通腑组优于潜阳熄风组和益气活血组,72 h各治疗组组间比较益气活血组优于化痰通腑组和潜阳熄风组;在升高PAI-1方面,72 h各组间比较益气活血组优于化痰通腑组和潜阳熄风组。结论:不同的中药复方可以降低vWF、t-PA血浆含量和升高PAI-1血浆含量抗脑缺血再灌注损伤,72 h益气活血方作用效果最佳。Objective：The present research was to investigate the optimal time of different TCM therapeutic methods by dynamically observing the changes of blood clotting and plasma change throughtesting blood clotting and plasma indieatrix and vWF, tPA, PAI-1 in the local cerebral ischemia-reperfusion （ I/R ） rats. Methods ： The local cerebral I/R model was established by ligation of the middle cerebral arteries. The animals were divided into the sham-operative group, the model group, the Yiqi Huoxue Recipe （ YHR ） group, the Zhenggan Xifeng Decoction （ ZXD ） group and the Huatan Tongfu Decoction （ HFD ） group.The levels of vWF, t-PA and PAI-1 were measured by enzyme-linked immunosorbent in plasma. Results ： The levels of vWF and t-PA were higher in model group than those in sham-operative group at all time points after I/R, and the level of PAI-1 was lower in model group than those in sham-operative group at all time points after I/tl .Compared with those in model, the levels of vWF and t-PA were lower in all treatment groups, the level of PAI-1 was higher in all treatment groups.The optimal effect was shown in ZXD group （ vWF ） or HFD group （ t-PA ） at 24 h, in HFD group （ vWF ） at 48 h, and in YHR groups （ vWF, t-PA and PAl-1 ） at 72 h after I/R. Conclusion ： The different traditional Chinese medicinal compounds reduce pathologic injury after focal cerebral I/R in rats by inhibiting levels of vWF and t-PA in plasma, and increasing pathologic injury after focal cerebral I/R in rats by inhibiting levels of PAI-1 in plasma, the optimal effect of YHR was shown at 72h after I/R.

关 键 词：脑缺血再灌注 益气活血 潜阳熄风 化痰通腑 血管性血友病因子 组织型纤溶酶原激活物 组织型纤溶酶原激活物抑制物 

分 类 号：R285[医药卫生—中药学]