商陆单细胞平板培养及色素高产细胞株的筛选  

CELL CULTURE AND SCREEN FOR HIGH YIELDING CELL SARAIN OF PIGMENT IN PHYTOLACCA AXINOSA ROXB.

作　　者：李景原[1] 王太霞[1] 杨相甫[1] 张晋豫[1] 李贺敏[2] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453002 [2]河南农业大学农学系,河南郑州450002

出　　处：《天然产物研究与开发》2000年第4期62-65,共4页Natural Product Research and Development

摘　　要：研究了细胞悬浮培养时间、培养方法和接种密度对商陆单细胞平板培养植板率的影响 ,建立了筛选色素高产细胞株的简单实用的方法。结果表明 :普通单细胞平板时培养法培养商陆单细胞的植板率很低 ,而以悬浮培养 14～ 2 1d的单细胞为材料 ,接种密度为 5× 10 3/ ml时 ,进行条件培养和看护培养 ,植板率达 11.0 2 %。根据细胞平板培养结果 ,可在显微镜下直接观察单细胞克隆的颜色 。Factors such as cell suspension culture time,the ways of plating and cell density,which affects the plating efficiency of Phytolacca axinosa Roxb.were studied in this paper.The results showed that plating at density of 5×10 3 cells/ml and with monocells seperated from 14～ 21 d cell,high plating efficiency could be botained either by conditional plate culture or by nursing plate culture.High yielding cell strain of pigment of Phytolacca axinosa Roxb.were screened out.

关 键 词：商陆 单细胞平板培养 高产细胞株 天然色素 

分 类 号：TQ611.2[化学工程—精细化工] Q943.1[生物学—植物学]