蝰科(Viperidae)蝮亚科(Crotalinae)线粒体12S rRBA基因序列分析及其系统发育  

Phylogenetic Relationships Among Viperidae, Crotalinae Based on Mitochondrial 125 rRNA Sequence Variations

作　　者：周继亮[1] 姚永刚[2] 黄美华 杨大同[3] 吕顺清[3] 张亚平[2] 

机构地区：[1]浙江大学医学院基础医学系,杭州310031 [2]中国科学院昆明动物研究所细胞与分子进化开放实验室,昆明650223 [3]中国科学院昆明动物研究所系统动物学研究室,昆明650223

出　　处：《Acta Genetica Sinica》2000年第4期283-289,共7页

基　　金：浙江省自然科学基金!C010307

摘　　要：对蝮亚科（蛇岛蝮Ｇｌｏｙｄｉｕｓ ｓｈｅｄａｏｅｎｓｉｓ Ｚｈａｏ、黑眉蝮Ｇｌｏｙｄｉｕｓ ｓａｘａｔｉｌｉｓ Ｅｍｅｌｉａｎｏｖ、乌苏 里蝮Ｇｌｏｙｄｉｕｓ ｕｓｓｕｒｒｉｅｎｓｉｓ　 Ｅｍｅｌｉａｎｏｖ、　竹叶青Ｔｒｉｍｅｒｅｓｕｒｕｓ 　ｓｔｅｊｎｅｇｅｒｉ 　Ｓｃｈｍｉｄｔ和分别来自不 同地区的尖吻蝮Ｄｅｉｎａｇｋｉｓｔｒｏｄｏｎ　ａｃｕｔｕｓ　Ｇｕｅｎｔｈｅｒ、短尾蝮Ｇｌｏｙｄｉｕｓ　ｂｒｅｖｉｃａｕｄｕｓ　Ｓｔｅｊｎｅｇｅｒ各 两条）６种蛇共８个个体测定、分析了约３７０ｂｐ线粒体１２Ｓ ｒＲＮＡ基因序列；以游蛇科链蛇属半 棱鳞链蛇Ｄｉｎｏｄｏｎ ｓｅｍｉｃａｒｉｎａｔｕｓ　序列为外群构建分子系统树。分子数据结果支持尖吻蝮形态 学的属级分类地位；提示蛇岛蝮位于黑眉蝮的蛇岛亚种分类地位，同时探讨了蛇岛蝮的起源 问题；并提示短尾蝮和乌苏里蝮同位于种级分类地位。This paper analyzed the phylogenetic relationships and classification of pit vipers (Viperidae, Crotalinae) based on mitochondrial 12S rRNA gene sequence variations. We have sequenced mtDNA 12S rRNA gene about 370bp fragment from Gloydius saxatilis Emelianov, Gloydius shedaoensis Zhao, Gloydius ussurriensis (Emelianov), Trimeresurus stejnegeri Schmidt and Deinagkistrodon acutus, Gloydius brevicaudus (Stejneger) from two different localities, respectively. Combined with the sequence of Dinodon semicarinatus from GenBank, we have constructed two molecular phylogenetic trees using both Maximum-parsimony analysis and Neighbor-joining. Our results support the following conclusions f (1) Deinagkistrodon is a valid genus; (2) we also discussed the origin of Gloydius shedaoensis and showed it is a subspecies of Gloydius saxatilis, (3) Gloydius brevicaudsu and Gloydius ussurriensis are classified two species.

关 键 词：蝮亚科 12SrRNA 序列分析 分子系统树 蝰科 

分 类 号：Q52[生物学—生物化学]