苜蓿高含硫氨基酸蛋白转基因植株再生  

Regeneration of HNP Transgenic Alfalfa Plants by Agrobacterium Mediated Gene Transfer

作　　者：吕德扬[1] 范云六[2] 俞梅敏[2] 唐顺学[1] 侯宁[1] 汪清 

机构地区：[1]中国科学院遗传研究所,北京100101 [2]中国农业科学院生物技术研究中心,北京100081

出　　处：《Acta Genetica Sinica》2000年第4期331-337,共7页

基　　金：国家八六三计划;国家自然科学基金!39770474

摘　　要：通过农杆菌介导法将高含硫氨基酸蛋白基因转入苜蓿，成功地诱导转基因植株再生。转化植株生长和发育良好。苜蓿子叶外植体是较理想的转化受体。冷凉湿润的环境条件是苜蓿移栽成活率高所必需的。Transgenic plants regenerated from cotyledons of M sativa L infected using Agrobacterium tumefaciens A281 with plasmid pBF649 containing a gene encoding protein of high sulfur-amino acid content (HNP) were obtained successfully. The plants grew and fertiled well in field. Cotyledon explants were better recipient for transformation of M sativa L. Environment of suitable temperature (15℃) and high humidity on high viability of the plants transplanted into soil were essential conditions.

关 键 词：苜蓿 HNP 基因转化 植株再生 子叶外植体 

分 类 号：Q789[生物学—分子生物学]