香菇L26的菌丝培养特性及系统发育学分析  

Study of the Mycelium Cultivation Characteristics and Phylogenetic Analysis Based on rDNA-ITS of Lentinula edodesL 26

作　　者：胥玲[1] 朱莉[1] 王璐[1] 温洪宇[1] 

机构地区：[1]江苏师范大学生命科学学院,江苏徐州221116

出　　处：《种子》2013年第6期5-8,共4页Seed

基　　金：江苏师范大学博士基金项目(编号:09 XLR 13);江苏师范大学实验室建设与管理研究项目(5)

摘　　要：对香菇L 26菌丝生长所需碳源、氮源、无机盐和酸碱度等因素进行了研究,结果表明,香菇L 26生长所需最佳碳源为玉米粉,菌丝生长速度为0.601 cm/d;最佳氮源为蛋白胨,菌丝生长速度为0.520 cm/d;当基础培养基中MgSO4.7 H2O浓度为0.5 g/L时,菌丝生长最佳,生长速度为0.636 cm/d;培养基初始pH=6.0时,菌丝生长速度最快为0.533cm/d。采用通用引物ITS 1与ITS 4对香菇L 26的rDNA-ITS基因序列进行扩增与测序,获得了757 bp的DNA序列(GenBank accession No.JX 205093),基于5.8 S rDNA与ITS区序列构建系统发育树,对其分类学地位进行了分析研究。Growth conditions of Lentinula edodes L 26 and phylogenetic analysis based on rDNA-ITS sequence were studied in this paper. The results showed that the best carbon source was corn flour （20 g/L） ,mycelium growth rate was 0.601 cm/d,The best nitrogen source was Peptone （2 g/L） ,mycelium growth rate was 0.520 cm per day;When MgSO4 · 7 H2O concentration was 0.5 g/L, mycelium growth rate was 0.636 cm/d;When pH was 6.0, mycelium growth rate was 0.533 cm/d. A 757 bp fragment （ GenBank accession No. JX 205093 ） of rDNA-ITS from Lentinula edodes L 26 was amplified by polymerase chain reaction （PCR） using a pair of usual primer ITS 1 and ITS 4 and phylogenetic tree was constructed by using neighbor-joining method based on 5.8 S rDNA and ITS sequence. The taxonomic status of L 26 was analyzed in the end.

关 键 词：培养特性 系统发育学 控制变量法 同源性比对 rDNA—ITS序列 

分 类 号：S646.12[农业科学—蔬菜学]