不同碘摄入量对大鼠甲状腺和肝肾组织碘分布影响  

Effects of iodine intake of different doses on distribution of iodine in thyroid, liver and kidney of rats

作　　者：郑芳[1] 陈红玉[1] 马超[1] 史凯斌[1] 叶艳[2] 林来祥[2] 张媛[1] 

机构地区：[1]天津医科大学基础医学院,天津300070 [2]天津医科大学代谢病医院内分泌研究所卫生部及激素与发育重点实验室

出　　处：《环境与健康杂志》2013年第6期492-494,F0003,共4页Journal of Environment and Health

基　　金：国家自然科学基金(30800954);天津医科大学基础医学院大学生科研基金(2011)

摘　　要：目的研究不同碘摄入量对大鼠甲状腺、肝、肾组织碘含量以及钠碘转运体(NIS)表达的影响。方法将断乳后1个月的SPF/VAF级健康Wistar大鼠按体重随机分为低碘组(LI,碘摄入量约为0.6μg/d),正常碘组(NI,碘摄入量约为6.0μg/d),高碘组(HI,碘摄入量约为600.0μg/d),每组10只,雌雄各半。饲养3个月后,收集尿液,采用过硫酸铵消化-砷铈催化分光光度法测定尿碘含量。采用碱灰化-砷铈催化分光光度法测定甲状腺、肝脏、肾脏组织碘含量,并采用免疫组化技术检测NIS蛋白的分布与表达。结果 LI、NI、HI组尿碘中位数分别为8.1、344.7、25 404.3μg/L,尿碘中位数随着饮食中碘含量的增加而增加。LI组三种组织碘含量均低于NI组,HI组均高于NI组(均P<0.05)。LI组肾脏、甲状腺碘含量高于肝脏(P<0.05),肾脏与甲状腺的碘含量差异无统计学意义(P>0.05);NI组肝脏、肾脏、甲状腺碘含量依次增加,三者之间差异均具有统计学意义(F=46.443,P<0.05);HI组甲状腺碘含量低于肾脏、肝脏(P<0.05),肾脏与肝脏比,碘含量有增高趋势,但差异无统计学意义(P>0.05)。与NI组比较,NIS在LI组甲状腺滤泡上皮细胞基底膜强表达,而在HI组表达较弱;肾脏组织中,HI组肾小管上皮细胞基底膜和胞浆均有较强表达,而LI组表达相对较弱;肝脏组织中,不同组大鼠NIS的表达均不明显。结论碘摄入量不同时大鼠体内甲状腺、肝脏、肾脏的碘分布存在差异,这与组织中NIS蛋白的表达不同有关。Objective To detect the effects of iodine intake of different doses on the distribution of iodine in rats thyroid, liver, kidney and the expression of sodium-iodide symporter （NIS）. Methods Wistar rats, weaning for one month, were randomly divided into three groups according to their body weight： low iodine （LI, iodine intake was 0.6 μg/d）, normal iodine （NI, iodine intake was 6.0 μg/d）, high iodine （HI, iodine intake was 600.0 μg/d）. The ratio of female to male was 1 ：1 .After three months of treatment, urine iodine was determined by As-Ce-Catalytic spectrophotometry. The iodine contents in thyroids, livers and kidneys were determined by alkaline ash arsenic-cerium catalytic spectrophotometry, the distribution of the NIS protein and its expression were observed by immunohistochemistry. Results The medians of urine iodine of the LI, NI, and HI groups were 8.1,344.7 and 25 404.3 μg/L respectively. The urine iodine and iodine intake showed the parallel magnification. Among thyroid, liver and kidney, the iodine content showed a significant difference （P〈0.05）, based on the comparison of LI group and HI group with NI group. In LI group, the iodine contents of kidney and thyroid were higher than that of liver （P〈0.05）. There was no obvious difference between kidney and thyroid （P〉0.05）. In NI group, the iodine contents among kidney, thyroid and liver showed significant differences （F=46.443 ,P〈0.05）. In HI group, thyroid＇s iodine content was lower than those of kidney and liver（P〈0.05）. Compared with liver, the kidney＇s iodine content had an increasing trend（P〉0.05）. Significant differences in the expression of NIS protein appeared in different tissues of rats in different groups with the immunohistochemical methods. In thyroid tissue, in comparison with the NI group, the NIS in the LI group of thyroid follicular epithelial basement membrane showed strong expression in a linear distribution, with weak expression in the HI group. In the kidneys, the NIS in

关 键 词：碘 钠碘转运体 甲状腺 肝脏 肾脏 

分 类 号：R994.6[医药卫生—毒理学]