大鼠肝大部分切除前热休克对热休克蛋白和磷酸酶的影响  

THE EFFECT OF HEAT SHOCK BEFORE RAT PARTIAL HEPATECTOMY ON HSC70/HSP68 EXPRESSION AND PHOSPHATASE ACTIVITIES

作　　者：徐存拴 卢爱灵[1] 夏民[1] 李效阳 李永辉[1] 赵绪永[1] 

机构地区：[1]河南师范大学生命科学学院,新乡453002

出　　处：《实验生物学报》2000年第1期1-11,共11页Acta Biologiae Experimentalis Sinica

基　　金：中法先进技术合作项目

摘　　要：定性和定量分析了在大鼠2/3肝切除前8b热休克(46℃,30min)处理(HS-PH)的肝再生期间(0—144h)保持性热休克蛋白70/诱导性热休克蛋白68(HSC70/HSP68)分布和含量变化、酸性磷酸酶(ACP)和碱性磷酸酶(AKP)分布、种类和活性变化。并把上述结果同只进行热休克(46℃,30min)(HS)和只进行2/3肝切除(PH)时这些分子的变化进行了比较。发现:三种处理均可提高ACP、AKP活性和HSC70/HSP68表达量,但它们的变化规律不同。进一步分析发现,HS-PH后ACP活性增强是与140kD酶活性增加有关,而AKP活性增强则与140和160—180kD的酶活性增加有关。根据实验结果推测,ACP、AKP和HSC70/HSP68均在肝细胞的热休克反应和肝再生中起作用;它们可能均参与这些过程中的信号传导,但ACP可能在启动肝细胞增殖中起主导作用,AKP和HSC70/HSP68可能在胞质分裂中起主导作用。The contribution and content of the continuous heat shock protein 70/induced heat shock protein 68 ( HSC70/HSP68 ), the contribution, variety and activity of acid phosphatases (ACP) and alkaline phosphatases (AKP) had been analysed qualitatively and quantitatively during the liver regeneration after 2/3 hepatectomy (PH) and HS (heat shock at 46℃ for 30 min, recovery for 8 h), which were compared with the results only by HS and only by PH. It was shown that the three kinds of treatment all can increase the activity of ACP, AKP and the expression of HSC70/HSP68, but with different change pat- tern. A further analysis show that after HS-PH the enhanced activity of ACP is related with that of 140 kD phosphatases, the enhanced activity of AKP is associated with that of 140 kD and 160-180 kD phosphatases. It can be reckoned from the results that ACP, AKP and HSC70/HSP68 all act on the heat shock response of hepatocyte and liver regeneration, and may take part in signal transduction in these processes, but ACP may play a dominant role in the start of hepatocyte multiplication, AKP and HSC70/HSP68 may play a dominant role in cytokineses.

关 键 词：肝再生 热休克蛋白 磷酸酶 热休克反应 调控 

分 类 号：Q51[生物学—生物化学] Q55