IL-1诱导小鼠胸腺上皮细胞(MTEC1)克隆分泌化学趋化因子  

THE CAPABILITY OF IL-1 IN THE INDUCTION OF CHEMOKINE PRODUCTION BY MURINE THYMIC EPITHELIAL CELL (MTEC1) CLONES

作　　者：董巍[1] 陈慰峰[2] 刘祝公[2] 庞学文[2] 苏树芸 

机构地区：[1]中国医学科学院试验动物研究所,北京100020 [2]北京医科大学免疫学系,北京100083

出　　处：《实验生物学报》2000年第1期35-40,共6页Acta Biologiae Experimentalis Sinica

基　　金：ICGEB(98/016)基金

摘　　要：通过将本室所建小鼠胸腺上皮细胞系MTEC1进行克隆,获得由单一细胞来源的12个MTEC1-DW细胞克隆,检测各克隆分泌IL-1,IL-6及CF活性,分析诱导MTEC1-DW细胞克隆分泌CF的因素。选择不分泌IL-1及CF的MTEC1-DW克隆,于细胞培养中加入外源IL-1或/及IL-6,分析其细胞培养液中CF活性;选择分泌高活性IL-1及CF的MTEC1-DW克隆,于细胞培养中加入抗IL-1mAb,阻断IL-1活性,分析其细胞培养液中CF活性。结果显示:IL-1能诱导MTEC1-DW细胞克隆分泌CF,IL-6的这种作用则很微弱。To analyze the capability of IL-1 and IL-6 in the induction of chemokine (CF) production by mouse thymus epithelial cell (MTEC1) clones, MTEC1 cells were cloned through one cell culture and individual cell clones were established in long term culture referred to as MTECl-DW clones. The constitutive production of IL-1, IL-6 and CF by MTECl-DW clones was evaluated and the patterns of the cytokine production determined. Addition of exogenous IL-1 or IL-6 or both to the cultures of those MTECl-DW clones that are unable to produce CF , and incubated for 2 days, then, to assess the chemotactic activity in the cell culture supernatants (SNs). In the opposite, addition of anti-IL-1 mAb(s) to the cultures of those MTECl-DW clones that can pro- duce IL-1 and CF to neutralize secreted IL-1, then, to test chemotactic activity in the SNs after 2-day incubation. The results showed that in the MTECl-DW clones which were unable to consti-tutively produce IL-1 or CF, addition of IL-1 could induce these cloned cells to produce CF with high chemotactic activity. By contrast, addition of anti-IL-1 mAb (s) to those MTECl-DW clones that constitutively produce IL-1 and CF could significantly inhibit them to produce CF. IL-6 only exhibited weak activity in the induction of CF production by those cloned cells. Therefore , in the cytokine network regulation, CF production is mainly induced by endogenously produced IL-1 in MTEC1 cells.

关 键 词：胸腺上皮细胞 IL-1 细胞克隆 化学趋化因子 分泌 

分 类 号：Q785[生物学—分子生物学]