小鼠单卵裂球体外培养及染色体制备  

Culture and Chromosome Preparation of Mouse's Single Blastomere in vitro

作　　者：李秀蓉[1] 陆长富[1] 范立青[1] 朱文斌 卢光琇[1] 

机构地区：[1]湖南医科大学生殖工程研究室,中国湖南长沙410078

出　　处：《生命科学研究》2000年第1期31-34,共4页Life Science Research

基　　金：国家自然科学基金青年基金!资助项目(39100060)

摘　　要：为了探索研究植入前胚胎染色体病 (包括平衡易位 )诊断的可行性方法 ,作者进行了单卵裂球体外培养、染色体制备及显带技术研究 .在 B2 培养基加血清进行培养的基础上 ,比较了在两种不同处理因素进行体外培养时小鼠单卵裂球增殖情况 .B2 培养基加血清再加输卵管包埋进行培养 ,其单卵裂球体外增殖率为 50 % ( 87/1 74) ,单卵裂球染色体制备成功率为2 7.6% ;B2 培养基加腹水过滤液进行培养 ,其单卵裂球体外增殖率为 33% ( 78/2 37) ,单卵裂球染色体制备成功率为 3% .It is reported that the mouses single blastomeres were cultured in vitro, then prepared their chromosome and chromosome banding. The aim of the work was to explore the reliable method on human preimplantation genetic diagnosis (PGD) especially chromosome diseases including chromosome translocation. The single blastomeres developed in vitro under different culture conditions were researched. The blastomeres development rate in vitro with a small fragment of mouse oviduct is 50%(87/174), and 48 of them with visualizing chromosomes; with filter abdomen is 33%(78/237), and 8 of late with visualizing chromosomes.

关 键 词：单卵裂球 染色体 PGD 体外培养 制备方法 

分 类 号：Q343[生物学—遗传学] R596.04[医药卫生—内科学]