抗菌肽D基因导入番茄及转基因植株的鉴定  

Transfer of Antibacterial Peptide D Gene into Tomato and Identification of Transgenic Plants

作　　者：田长恩[1] 王正询 陈韬 周玉萍 黄自然[2] 黄亚东[2] 

机构地区：[1]广州师范学院生物学系,广州510405 [2]华南农业大学蚕桑系,广州510642

出　　处：《遗传》2000年第2期86-89,共4页Hereditas（Beijing)

基　　金：广州市重点项目 !(96-2-04-02)资助

摘　　要：用花粉管通道法将柞蚕抗菌肽D基因导入番茄 ,获得了转基因植株。应用PCR技术、DNA斑点杂交以及South ern印迹分析表明 ,抗菌肽D基因已整合到番茄基因组 ;而且 ,位于目的基因下游的胭脂碱合成酶基因有表达。青枯假单孢菌活体接种结合高发病大田种植试验结果显示 ,部分转基因植株的子一代具有较强的抗青枯病能力。By using pollen tube pathway,antibacterial peptide D(AP D) gene developed from Chinese oak silkwarm was transferred into tomato.PCR,dot and Southern blot analysis confirmed that stable integration of AP D gene into the tomato genome had occurred.The expression of NOP gene which was localized at downstream of AP D gene was confirmed with nopaline detection.In comparision with non transgenicplants,some transgenic plant expressed a stronger resistance value to bacterialwilt disease (Pseudomonas solanacearum).

关 键 词：抗菌肽D基因 花粉通道法 番茄 青枯病 抗病育种 

分 类 号：S188[农业科学—农业基础科学]