低水平乙型肝炎病毒表面抗原S区序列分析  

Analysis of Low levels of hepatitis B virus surface antigen S region sequence

作　　者：计玉仙[1] 张志明[1] 李建平[1] 姜小建[1] 肖改娥[1] 马莹[1] 舒放[1] 姜立茹[1] 王鑫[1] 

机构地区：[1]西安市中心医院检验科,陕西西安710003

出　　处：《中国实验诊断学》2013年第6期1022-1025,共4页Chinese Journal of Laboratory Diagnosis

基　　金：陕西省社会发展攻关计划项目(2011K12-01-05)

摘　　要：目的对低水平乙型肝炎病毒表面抗原进行病毒S区基因分析。方法利用套式PCR法(Nested PCR)特异性扩增乙型肝炎病毒DNA的S区,对PCR扩增产物直接进行DNA序列分析,测序结果经Genotyping软件分型并与美国GeneBank中基因序列进行BLAST比对。结果 37例低水平乙型肝炎病毒表面抗原标本中,成功扩增并测序32份,其余5份扩增产物因不满足测序要求无法进行S区序列分析,32份成功测序标本经Genotyping分型,B型20例,C型12例,分别与GeneBank中收录的AF100309、AB014381同源性最高;血清学分型18例为adr,10例为adw,3例为ayw,1例为ayr,并发现21例S区核酸序列发生点突变,其中15例突变不会引起编码抗原决定簇氨基酸突变,为无义突变,而其他6例点突变均可引起抗原决定族编码的氨基酸的改变,从而导致表面抗原结构改变。结论对低水平乙型肝炎病毒表面抗原患者S区序列研究将为低水平乙型肝炎发病机制、流行病学以及个体化治疗奠定理论基础。Objective To analysis low levels of hepatitis B virus surface antigen virus S gene.Methods Using nested PCR（Nested PCR） and specificity amplification of hepatitis B virus DNA S area,PCR amplification products direct DNA sequence analysis,DNA sequencing results by Genotyping software typing and the United States GeneBank gene sequences of BLAST ratio.Results 37 cases of low levels of hepatitis B virus surface antigen in specimens,successful amplification and sequencing of 32,the remaining 5 amplification product does not meet the requirements for sequencing to S region sequence analysis,32 successful sequencing specimens by Genotyping typing,type B in 20 cases,12 cases of type C,and GeneBank published in AF100309、AB014381 homologous highest;serum type ADR in 18 cases,10 cases were ADW,3 cases were ayw,1 cases were Ayr,and found 21 cases of S nucleic acid sequence point mutations,including 15 cases of mutation does not cause encoding antigenic determinants for amino acid mutations,nonsense mutations,while the other 6 cases with point mutation may cause epitopes encoded amino acid changes,thereby causing the surface antigen structure change.Conclusion For low levels of hepatitis B virus surface antigen in patients with S region sequence research for low level hepatitis Mechanism,epidemiology and individualized treatment to lay the theoretical foundation.

关 键 词：乙型肝炎表面抗原 S区 基因 聚合酶链反应 

分 类 号：R512.62[医药卫生—内科学]