大豆甙元联合TRAIL诱导胃癌SGC-7901细胞凋亡的机制研究  

Research the mechanism of apoptosis induced by Daidzein combined with TRAIL in SGC-7901 cells

作　　者：杜志敏[1] 杨静静[1] 马倩[1] 王天云[2] 李齐印[3] 徐志强[4] 李万里[1] 

机构地区：[1]新乡医学院免疫教研室,新乡453003 [2]新乡医学院生物化学研究中心,新乡453003 [3]新乡医学院基础医学院,新乡453003 [4]新乡医学院三全学院,新乡453003

出　　处：《中国免疫学杂志》2013年第6期570-574,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金项目(30970055C010202);河南省新乡医学院高学历人才科研启动资助项目(100179)资助

摘　　要：目的:研究大豆甙元(Daidzein,Da)联合TRAIL对SGC-7901细胞的影响。方法:采用不同浓度的Da和TRAIL单独及联合作用于SGC-7901细胞,通过MTT细胞和流式细胞术检测增殖、凋亡和周期的变化情况,透射电镜观察对细胞内部形态和微结构的影响,利用Western blot检测对相关凋亡基因Bcl-2、survivin、caspase-3的表达变化。结果:Da联合TRAIL处理人胃癌SGC-7901细胞后,生长有明显的抑制作用,并且具有一定的剂量依赖性;细胞周期阻滞于S期;细胞内部出现典型凋亡形态学改变;Bcl-2和survivin蛋白表达下调,caspase-3蛋白被上调。结论:Da联合TRAIL通过细胞周期S期阻滞,及下调Bcl-2、survivin蛋白及增强caspase3蛋白表达而诱导胃癌细胞凋亡。Objective：To investigate the effects of Daidzein combined with TRAIL in gastric adenocarcinoma cell SGC- 7901. Methods： SGC-7901 cells were treated with Da or/and TRAIL at different concentration for indicated times. MTY assays were per- formed to measure the growth inhibitory effect of Da or/andTRAIL. Cell cycle and apoptosis of cells were analyzed using flow cytome- try. Transmission electron microscope was introduced to Observe the changes of internal morphology and microstructure of SGC-7901 cells. Western blot was used to examine the changes of Bcl-2, caspase-3, survivin expression. Results： Da combined with TRAIL obvi- ously inhibits the cell growth of SGC-7901 cells in a doseTdependent manner and induces cell cycle arrest in S phase. Typical apoptosis morphology changes were observed after the combined treatment. Bcl-2 and survivin proteins were decreased and the level of caspase-3 was increased upon the combined treatment. Conclusion：Da combined with TRAIL lead to apoptosis of gastric adenocarcinoma cells through induction of cell cycle arrest in S phase, inhibition.of Bcl-2 and survivin protein expression, and enhancement of caspase-3 protein level.

关 键 词：大豆甙元(Da) TRAIL 胃癌SGC-7901细胞 细胞凋亡 

分 类 号：R392.11[医药卫生—免疫学]