两例罕见的血小板抗原HPA-21bw等位基因报告  

Two cases report of rare platelet antigen HPA-21bw allele

作　　者：周豪杰[1] 聂咏梅[1] 李晓帆[1] 汪传喜[1] 

机构地区：[1]广州血液中心,广州510095

出　　处：《中国免疫学杂志》2013年第6期622-624,共3页Chinese Journal of Immunology

基　　金：广州市医药卫生科技项目(No.20131A010023)

摘　　要：目的:调查中国人群人类血小板抗原HPA-21w多态性。方法:采用聚合酶链反应-序列特异性引物法(PCR-SSP)基因分型技术,对广州汉族血小板献血者进行HPA-21w基因分型,并使用PCR扩增HPA-21w基因片段,进行DNA测序验证。结果:在200例受检者中发现2例HPA-21a/b杂合子个体,DNA测序表明GPIIIa编码基因1960位G→A,导致GPIIIa膜糖蛋白第628位谷氨酸被赖氨酸所取代,产生HPA-21bw抗原特异性。中国人群HPA-21bw的等位基因频率为0.50%。结论:在中国人群首次检出HPA-21bw等位基因,提示在血小板同种免疫症的诊断和血小板输注治疗中,该抗原具有免疫学意义。Objective：To study the polymorphism of human platelet antigen HPA-21w in the Chinese population. Methods： A total of 200 Han voluntary platelet donors in Guangzhou were genotyped for HPA-21w by using a polymerase chain reaction-sequence specific primers （PCR-SSP） method. In order to confirm the genotyping result, a fragment of HPA-21w gene was amplified and then sequenced directly. Results： Two individuals with a rare HPA-21a/b genotype were found by using PCR-SSP method. DNA-sequen- cing showed that a single G to A substitution at nucleotide 1960 occurred, resulting in the replacement of a glutamic acid with a lysine acid at position 628 of GPIIIa protein. This substitution generated antigenic specificity HPA-21 bw. The allele frequency of HPA-21 bw was 0.50% in the Chinese population. Conclusion： This is the first study reported the rare HPA-21bw allele in the Chinese popula- tion. The detection of two rare HPA-21 bw alleles suggests that HPA-21 bw should he considered in platelet alloimmunization syndromes and platelet transfusion therapy.

关 键 词：中国人群 HPA-21w抗原 PCR-SSP DNA测序 

分 类 号：R331.143[医药卫生—人体生理学]