咳喘宁口服液对支气管哮喘大鼠PI3K信号转导途径的影响  

Effect of Kechuanning Oral Liquid on Signal Transduction Pathway of PI3K in Asthma Rats Induced by Virus

作　　者：罗银河[1] 李华[1] 杨静宜[2] 王孟清[2] 黄胜[2] 黄婷[2] 蒋屏[2] 焦珞珈[1] 李英[1] 

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]湖南中医药大学第一附属医院,湖南长沙410007

出　　处：《中国中医药信息杂志》2013年第7期30-32,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学青年基金(30901921)

摘　　要：目的观察咳喘宁口服液对呼吸道合胞病毒诱发支气管哮喘(以下简称"哮喘")大鼠CD4+、CD8+T细胞内磷脂酰肌醇-3-激酶(PI3K)-110α表达的影响,探讨其防治病毒诱发哮喘的机理。方法将SD大鼠随机分为磷酸盐缓冲液(PBS)对照组、模型组、地塞米松+沙丁胺醇组(阳性药组)和咳喘宁组。建立卵白蛋白哮喘大鼠模型,并以呼吸道合胞病毒激发哮喘。采用磁珠结合法分离CD4+、CD8+T细胞,使用流式细胞仪检测T细胞内PI3K-110α的表达水平。结果模型组大鼠CD4+、CD8+T细胞百分率及CD4+、CD8+T细胞内PI3K-110α的表达水平显著高于PBS对照组、阳性药组及咳喘宁组,差异有统计学意义(均P<0.01)。经Pearson相关分析,各组大鼠CD4+、CD8+T细胞百分率与CD4+、CD8+T细胞内表达的PI3K-110α的表达水平无明显相关性。结论 CD4+、CD8+T细胞参与了哮喘免疫平衡调节,其表达的PI3K在病毒诱发哮喘模型中明显增高。咳喘宁口服液能抑制CD4+、CD8+T细胞内PI3K的表达,可能是其治疗哮喘的免疫机制之一。Objective To observe the effect of Kechuanning Oral liquid on the expression of P13K in CD4＋ and CD8＋T cells of asthma rats induced by respiratory syncytial virus（RSV）,and explore its mechanism of preventing and treating asthma induced by virus.Methods SD rats were randomly divided into phosphate-buffered saline（PBS） control group,model group,dexamethasone and salbutamol group（positive drug group）,and Kechuanning oral liquid group.Ovalbumin asthma rats model was built and induced by RSV.CD4＋ and CD8＋T cells were separated by magnetic beads method.The expressive level of PI3K-110α in T cells was detected by immumofluorescence method with flow cytometry.Results Compared with PBS control group,positive drug group and Kechuanning oral liquid group,the CD4＋ and CD8＋T cells percentage and the expression level of PI3K-110α in CD4＋ and CD8＋T cells of model group were significantly higher.The differences were statistically significant（P 0.01）.The CD4＋ and CD8＋T cells percentage was not associated with the expression level of PI3K-110α in CD4＋ and CD8＋T cells of each group by Pearson correlation analysis.Conclusion The CD4＋ and CD8＋T cells involve in the asthma immunity balanced modulation,and the level of PI3K expressed by CD4＋ and CD8＋T cells increase distinctly.Kechuanning oral liquid can restrain the expression of PI3K in CD4＋ and CD8＋T cells,which might be one of the immunologic mechanisms of treating asthma.

关 键 词：支气管哮喘 磷脂酰肌醇-3-激酶信号转导 咳喘宁口服液 细胞培养 大鼠 

分 类 号：R285.5[医药卫生—中药学]