电针内关对心肌缺血大鼠经穴皮肤组织中环磷酸鸟苷的影响  

Effect on cGMP of Meridian Skin with Electroacupuncture on Myocardial Ischemia Rats

作　　者：丁娜[1] 李晓泓[1] 嵇波[1] 胡琅琳[1] 孙志芳[1] 朱世鹏[1] 罗丽 

机构地区：[1]北京中医药大学针灸推拿学院,北京100029

出　　处：《中国中医药信息杂志》2013年第7期46-48,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81072860);北京中医药大学自主选题项目(01302)

摘　　要：目的观察大鼠心肌缺血损伤后,经穴皮肤组织中环磷酸鸟苷(cGMP)的特异性变化及电针"内关"对其的干予作用。方法 6只雄性Wistar大鼠作为正常组,18只大鼠制备心肌缺血后,分为模型组、高频组、低频组,高频组和低频组分别予100、2 Hz电针双侧"内关",针刺时间20 min。治疗完毕后采用酶联免疫吸附法检测"内关"、"天泉"、"外关"及"足三里"等穴区皮肤组织中cGMP的含量。结果心肌缺血模型组的"内关"、"天泉"、"外关"及"足三里"穴区cGMP含量较正常组均显著升高(P<0.05或P<0.001);而高频或低频电针"内关"后,上述穴区cGMP含量较模型组均显著降低(P<0.05或P<0.001)。结论高频或低频电针"内关"可保护大鼠心肌缺血损伤,其机制可能与降低心脏相关经脉穴区cGMP含量相关。Objective To observe the specific changes of content of cyclic guanosine monophosphate（cGMP） around meridian skin and specific influences of electroacupunture Neiguan（PC6） on cGMP of rats with acute myocardial ischemia（AMI）.Methods With 6 male Wistar rats as normal group,18 rats were made AMI model and divided into model group,high-frequency group and low-frequency group（n=6）.The high-frequency and low-frequency group were given electroacupuncture in bilateral PC6 with 100 and 2 Hz for 20 min respectively.The content of cGMP in skin around PC6,Ximen（PC4）,Tianquan（PC2）,Waiguan（TE5） and Zusanli（ST36） was detected by using enzyme-linked immunosorbent method after treatment.Results In model group,cGMP content increased significantly around PC6,PC2,TE5 and ST36 compared with normal group（P 0.05,P 0.001）.After the treatment with high-frequency or low-frequency electroacupunture in PC6,cGMP content decreased significantly in skin around PC6,PC2,TE5 and ST36 compared with model group（P 0.05,P 0.001）.Conclusion High-frequency electroacupunture or high-frequency electroacupunture used in PC6 can protect rats from the injury of AMI.The mechanism may be related to the decrease of cGMP content around the acupoint areas in heart-related meridian.

关 键 词：心肌缺血 电针 内关 环磷酸鸟苷 大鼠 

分 类 号：R245.3[医药卫生—针灸推拿学]