抗大麦黄矮病毒小麦-中间偃麦草二体异代换系的选育和细胞、生化、分子生物学鉴定  

Creation and Cytological, Biochemical, Molecular Identification of Alien Disomic Substitution Lines with BYDV-resistance from Triticum aestivum-Agropyron intermedium Hybrids

作　　者：唐顺学[1] 李义文[1] 梁辉[1] 曲乐庆[1] 白建荣[1] 贾双娥[1] 魏晓丽[1] 李振声[1] 贾旭[1] B.FRIEBE 

机构地区：[1]中国科学院遗传研究所植物细胞与染色体工程国家重点实验室 [2]Department of Pathology Kansas University,Manhattan KS66506_5501,USA

出　　处：《Acta Botanica Sinica》2000年第9期952-956,共5页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家自然科学基金资助项目!( 3 9770 5 17)&&

摘　　要：结合花药培养和常规选育 ,从 77_5 433×中 5杂交组合后代中选育出 6个抗大麦黄矮病毒 (barleyyellowdwarfvirus ,BYDV)、染色体数目为 42的小麦 (TriticumaestivumL .)新种质 ,并用减数分裂配对分析、单体小麦测交法、基因组原位杂交、C分带、同工酶等电聚焦和RAPD对上述材料进行了综合鉴定 ;表明这些材料都是小麦_中间偃麦草(AgropyronintermediumGarten)二体异代换系 ,其抗病性来自其携带的一对中间偃麦草染色体 (来源于中 5 )。By the method of combined anther culture and conventional selection, six new BYDV (barley yellow dwarf virus)_resistant wheat germplasms (2n=42) were obtained from the crosses between common wheat cv. 77_5433 and Zhong 5, a partial Triticum aestivum_Agropyron intermedium amphiploid. The six germplasms were comprehensively identified by meiosis pairing analysis, testing cross analysis using monosomic analysis, C_banding, isoelectrofocusing (IEF) of isoenzyme and RAPD analyses. The results indicated that all the germplasms were alien substitution lines carrying resistant genes originated from Zhong 5.

关 键 词：BYDV 抗病种质 二体异代换系 小麦 抗性鉴定 

分 类 号：Q943.1[生物学—植物学]