脂肪酸多相脂质体与癌细胞膜相互作用的ESR谱研究  

Study on the Interaction Between the Liposomes Containing Unsaturated Fatty Acid and Cancer Cell Membrane by the Technique of ESR.

作　　者：孙润广[1] 张静[2] 王永昌[1] pub.xaonline.sn.cn 

机构地区：[1]西安交通大学理学院,西安710049 [2]陕西师范大学实验中心,西安710062

出　　处：《生物化学与生物物理进展》2000年第3期296-300,共5页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金资助项目 !(2 96 72 0 42 ) ;西安交通大学博士论文基金资助项目! (DFXJU 1 998 9)

摘　　要：用电子自旋共振 (ESR)技术对液晶态多烯脂肪酸多相脂质体与癌细胞膜相互作用进行了研究 .并探讨了其在抑制和杀伤癌细胞过程中可能具有的生物学意义 .实验发现 :油酸多相脂质体的影响使自旋标记物在Ec腹水肝癌细胞膜上的强固定化作用减弱 ,弱固定化作用增强 ,使自旋标记物运动自由度增加 .亚油酸多相脂质体的影响使自旋标记物在乳腺癌细胞膜上的强固定化作用增强 ,弱固定化作用减弱 ,使自旋标记物运动自由度受到限制 .蓖麻酸多相脂质体的影响使自旋标记物在S180 实体瘤细胞膜上的强固定化作用增强 ,弱固定化作用减弱 ,使自旋标记物运动自由度受到限制 .结果表明 。A study by the technique of electron spin resonance (ESR) has been made on the interaction between the liposomes containing unsaturated fatty acid and cancer cell membrane, and probed into that they have may be biological significance in damaged and inhibited cancer cells. Experimental results showed following: the effect of the oleic heterogeneous liposomes causes that the powerful fixed effect of the spin probe is weakened that the weak fixed effect of the spin probe is strengthened, and that the free direction of the spin probe movement is increased. The effect of linoleic heterogeneous liposomes causes that the powerful fixed effect of the spin probe is strengthened, and the weak fixed effect of the spin probe was weakened on the membrane of mammary tumor, and that the free direction of the spin probe movement is impeded. The effect of ricinoleic heterogeneous liposomes is the same with linoleic heterogeneous liposomes on the neoplasm cell. The result indicates that the unsaturated fatty acid can react with mercaptan groups and change the conformations of membrane proteins of tumor cells.

关 键 词：脂质体 多烯脂肪酸 抗癌药物 ESR谱 癌细胞膜 

分 类 号：R979.1[医药卫生—药品] R963[医药卫生—药学]