一步法从人血浆中制备天然血管生成抑制素  

Single-step Method to Prepare Native Angiostatin From Human Plasma.

作　　者：李福洋[1] 何鹏[1] 刘新平[1] 张英起[2] 杨静华[3] 樊代明[3] 药立波[1] fmmu.edu.cn 

机构地区：[1]第四军医大学生物化学与分子生物学教研室,西安710032 [2]第四军医大学生物技术中心 [3]第四军医大学西京医院消化病研究所

出　　处：《生物化学与生物物理进展》2000年第3期325-327,共3页Progress In Biochemistry and Biophysics

基　　金：国家杰出青年自然科学基金资助课题! (3 982 5 1 1 3 ;3 96 2 5 0 3 )

摘　　要：血管生成抑制素 (angiostatin)能特异地抑制新生血管生成 ,有潜在的临床应用价值 .利用亲和层析从人血浆中纯化纤溶酶原 ,并在原位进行弹性蛋白酶有限消化产生angiostatin片段 ,经过洗涤 ,然后用 0 2mol/L 6 氨基己酸溶液将angiostatin特异性洗脱 .此改进使整个制备过程变得简单、快速和高效 .As a specific angiogenesis inhibitor, angiostatin can inhibit new vascular formation, and it has potential clinical practical value. Human plasminogen was purified from human plasma by affinity chromatograph, and then in situ digestion of plasminogen by elastase was carried out to produce angiostatin fragments. After washing, angiostain was eluted specifically by 0 2 mol/L 6 aminocaproic acid solution. This improvement made this method be simpler, rapider and more efficient. In vitro and in vivo experiments indicated that this purified protein has potent antiangiogenic activity.

关 键 词：血管生成抑制素 抗肿瘤转移 一步亲和层析 血浆 

分 类 号：R979.1[医药卫生—药品] TQ464.7[医药卫生—药学]