冬小麦原生质体培养的胚状体直接发生  

Direct Embryogenesis from Protoplast of Winter Wheat

作　　者：葛台明[1] 章荣德[2] 秦发兰[2] 余毓君[2] 谢岳峰[2] 

机构地区：[1]湖北民族学院园艺系,恩施445000 [2]华中农业大学作物遗传改良国家重点实验室,武汉430070

出　　处：《生物工程学报》2000年第5期609-613,共5页Chinese Journal of Biotechnology

基　　金：华中农业大学作物遗传改良国家重点实验室开放项目资助;湖北省教委青年项目

摘　　要：冬小麦品种“京花一号”胚性愈伤组织在改良的N6培养基 (NBD培养基 )上继代得到易碎型胚性愈伤组织 ,转入改良MS液体培养基 (MSDL培养基 )后得到胚性悬浮系 ,分离的原生质体在改良的MS培养基 (MSDP培养基 )上培养 ,再生细胞直接产生体细胞胚胎 ,并再生出完整植株。体细胞胚胎形成过程与小麦合子胚的形成过程十分相似。Friable embryogenic calli were obtained on a modified N 6 medium (NBD medium) from a winter wheat cultivar “Jinghua No.1”( Triticum aestivum L. cv. Jinghua No.1) and were transferred to a modified MS liquid medium (MSDL medium) to initiate embryogenic suspension cultures. Protoplasts were isolated from the suspensions and cultured on a modified MS medium (MSDP medium). The somatic embryoids were formed directly from the protoplasts and germinated into entire plants. The development of the somatic embryoids was very similar to that of zygotic embryos of wheat.

关 键 词：冬小麦 原生质体培养 体细胞胚发生 植株再生 

分 类 号：Q943[生物学—植物学]