聚球藻7002在光生物反应器中的光自养培养  

Photoautotrophic Cultivation of Synechococcus sp. PCC7002 in Photobioreactor

作　　者：康瑞娟[1] 周文齐 蔡昭铃[1] 施定基[2] 

机构地区：[1]中国科学院化工冶金研究所,生化工程国家重点实验室,北京100080 [2]中国科学院植物研究所,北京100093

出　　处：《生物工程学报》2000年第5期618-622,共5页Chinese Journal of Biotechnology

摘　　要：通过对聚球藻 70 0 2在光生物反应器中的培养 ,研究了光强在聚球藻 70 0 2培养液中的衰减规律 ,得到了培养过程光强随藻细胞浓度和光程距离变化的关系式 ,即I=I0 exp[- (- 0 0 2 39+0 0 777OD750 )·L]。并对培养过程特性及培养温度、外加CO2 浓度和光照强度对藻细胞生长的影响进行了较为详细的研究 ,得到了反应器中较为适宜的聚球藻 70 0 2的培养条件 ,藻细胞培养密度达到 3 4g/L(干重 ) ,体积产率达到 0 5 7g/ (L·d)的较高水平。The Photoautotrophic cultivation of Synechococcus sp.PCC 7002 in a 15L\|airlift photobioreactor was carried out.The changes of light intensity with cell density and optical length in the cultivation system were investigated.Based on experimental results,the light attenuation could be described by I=I 0exp [-(-0 0239+0 0777 OD 750 )·L].The effects of the variations of light intensity\,CO 2 concentration in gas inlet and culture temperature on the growth of cells during the cultivation process have also been studied.The optimized condition was determined and a high dry cell density of 3\^4g/L was obtained.The volumetric productivity reached 0\^57g/(L·d) under the optimized condition.

关 键 词：聚球藻7002 光生物反应器 光自养培养 光衰减 

分 类 号：Q943[生物学—植物学]