人血小板生成素cDNA转导促进小鼠生长与胰岛素样生长因子相关  

Human Thrombopoietin cDNA Transfer Promotes Mice Growth Associated with an Insulin-like Growth Factor

作　　者：赵建增 陈立敏[1] 梅英杰[1] 张国荣[1] 王晓平[1] 

机构地区：[1]空军总医院基因治疗研究室,北京100036

出　　处：《中国生物化学与分子生物学报》2000年第4期524-528,共5页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金!资助 ( 3 9870 814 )

摘　　要：人血小板生成素 ( TPO)基因转导能刺激小鼠生长 .将编码人 TPO的真核表达质粒pc DNA3 h TPO、野生型 pc DNA3 或生理盐水注射小鼠后肢肌肉 ,称量小鼠的体重、器官重 .用半定量 ELISA检测 TPO转导小鼠血清中胰岛素样生长因子 ( IGF) - 、 和血小板源生长因子( PDGF)水平 .结果发现 ,TPO基因转导能促进小鼠的生长 .这种促生长作用与年龄和性别有关 ,但与动物的种系无关 .8～ 1 2周龄雌鼠在基因转导后 1周生长开始加快 .在第 1、2、3和 4周的升高幅度分别为 4.3%、5.3%、4.1 %和 4.38% ( P分别 <0 .0 0 5、0 .0 0 2、0 .0 1和 0 .0 1 ) .小于 4周龄的幼鼠、大于 1 6周的老龄鼠 ,以及雄性鼠在基因转导后体重无明显改变 .半定量 ELISA分析发现 ,体重增加的成年雌性鼠 ,其血清中 IGFs和 PDGF均升高 .基因转导鼠血液中葡萄糖、甘油三酯及碱性磷酸酶水平升高 .结果表明 ,一些生长因子 ,特别是 IGF- ,介导着 TPO对转基因动物的生长刺激作用 .To study the effect of thrombopoietin(TPO) gene delivery on the growth of mice,human TPO plasmid DNA encapsulated with lipofectin was injected into mouse muscle.And female mice of 8-12 weeks old were found to have a significent body weight gain compared with the controls following the 1st,2nd,3rd and 4th week( P <0 005,0 002,0 01 and 0 01 respectively),which had a characteristic of rapid growth ( P <0 01) in liver and spleen.However,the change of body weight in such mice injected with TPO cDNA as the males of the same age,the young ones of less than 4 weeks or the females of more than 16 weeks were not significantly different.Semi\|quantitative ELISA analysis demonstrated that the serum levels of platelet derived growth factor(PDGF),insulin\|like growth factors(IGF\|)Ⅰ and Ⅱ in the delivered mice rose correspondingly.Serum glucose,triglyceride,and alkaline phosphatase were also increased.It is suggested that some other growth factors,especially IGF\|1,mediate part of in vivo activities of TPO.

关 键 词：血小板生成素 基因转导 促生长 胰岛素样生长因 

分 类 号：Q593.5[生物学—生物化学] Q26