用免疫共沉淀进一步确定SMAD4中间连接区在形成同源和异源复合物时的作用  

Further Identification of the Function of SMAD4 Linker Region in Homomeric and Heteromeric Complexes with Co-immunoprecipitation

作　　者：刘一平[1] 邓继先[1] 张英丽[2] 吕娅歆[1] 程萱[1] 黄翠芬[1] 

机构地区：[1]军事医学科学院生物工程研究所,北京100071 [2]白求恩医科大学第三临床医院

出　　处：《中国生物化学与分子生物学报》2000年第4期537-541,共5页Chinese Journal of Biochemistry and Molecular Biology

摘　　要：用酵母双杂交系统发现 SMAD4的中间连接区能与 SMAD3相互作用 ,而 SMAD4的 N区和 C区不能与 SMAD3相互作用 ,此结果与前人报道的结果有出入 .用细胞免疫共沉淀的方法进一步证实此现象 .结果与酵母双杂交的结果完全吻合 .说明 SMAD4与 SMAD3相互作用形成异源复合物时确实是通过It is suggested that the linker region of SMAD4 has no function in the heteromeric and homomeric interaction of SMAD4.But in the previous research with yeast two\|hybrid assay,it was observed that the linker region of SMAD4 played a key role in the heteromeric interactions of SMAD4 and SMAD3,and in the homomeric interaction of SMAD4.It verified the function of SMAD4 linker region in heteromeric and homomeric interaction with co\|immunoprecipitation.The result showed that the SMAD4 linker region did play essential role in the interactions.

关 键 词：SMAD4 SMAD3 免疫共沉淀 中间连接区 细胞内转导 

分 类 号：Q257[生物学—细胞生物学] Q78