甘草水溶物诱导MGC-803细胞凋亡中胞内Ca^(2+)、pH与线粒体△ψ_m的变化  

Changes of Intracellular Ca^(2+) ,pH Value and Mitochondria △Ψ_m in the Apoptosis of MGC-803 Cells Induced by Glycyrrhiza uralensis Fisch

作　　者：马靖[1] 李玉梅[2] 庞大本[2] 徐安龙[1] 

机构地区：[1]中山大学生命科学学院生物化学系,广州510275 [2]中国中医研究院基础理论研究所,北京100700

出　　处：《中国生物化学与分子生物学报》2000年第4期556-558,共3页Chinese Journal of Biochemistry and Molecular Biology

基　　金：广州市科委重点项目! ( 97-Z-12 -0 1);国家新药博士基金!( 96-90 1-0 6-3 6)

摘　　要：Changes of intracellular Ca 2+ ,pH value and mitochondria membrane potential(△Ψ m) in the apoptosis of MGC 803 cells induced by water soluble constituents of Glycyrrhiza uralensis Fisch(WSCG) were investigated.MGC 803 cells were incubated with 0.5,0.75,1.0 and 1.5 g/L WSCG for 1,4,7,11,15,19 and 23 hours respectively.The percentage of apoptosis and intracellular Ca 2+ content increased in a dose and time dependent manner,except that the intracellular Ca 2+ content of 1.5 g/L WSCG treated cells started to decrease after 11 hours.The cells were alkalized after various treatments,except that 1.5 g/L WSCG treated cells turned to acidify after 11 hours.It was found that the mitochondria △Ψ m of all treated cells decreased drastically at the first hour,then kept decreasing slowly until the 23rd hour.The results indicated that intracellular Ca 2+ ,pH and mitochondria △Ψ m all played pivotal roles in the apoptosis of MGC 803 cells induced by WSCG.Changes of intracellular Ca 2+ ,pH value and mitochondria membrane potential(△Ψ m) in the apoptosis of MGC 803 cells induced by water soluble constituents of Glycyrrhiza uralensis Fisch(WSCG) were investigated.MGC 803 cells were incubated with 0.5,0.75,1.0 and 1.5 g/L WSCG for 1,4,7,11,15,19 and 23 hours respectively.The percentage of apoptosis and intracellular Ca 2+ content increased in a dose and time dependent manner,except that the intracellular Ca 2+ content of 1.5 g/L WSCG treated cells started to decrease after 11 hours.The cells were alkalized after various treatments,except that 1.5 g/L WSCG treated cells turned to acidify after 11 hours.It was found that the mitochondria △Ψ m of all treated cells decreased drastically at the first hour,then kept decreasing slowly until the 23rd hour.The results indicated that intracellular Ca 2+ ,pH and mitochondria △Ψ m all played pivotal roles in the apoptosis of MGC 803 cells induced by WSCG.

关 键 词：甘草 细胞凋亡 胞内CA^2+ 胞内PH 线粒体△Ψm 

分 类 号：Q255[生物学—细胞生物学] Q27