差异显示法克隆与小麦低温诱导相关的磷脂酰乙醇胺结合蛋白基因  

Cloning of a Phosphatidyl Ethanolamine Binding Protein Gene Associated with Cold-induced in Wheat by mRNA Differential Display Method

作　　者：刘瑞芬[1,2] 余东[2,3] 孙学武[1,2] 谭炎宁[2,3,4] 孙志忠[2,3] 袁贵龙[2,3] 段美娟[1,2,3,4] 袁定阳[1,2,3] 

机构地区：[1]中南大学隆平分院,长沙410125 [2]湖南杂交水稻研究中心,长沙410125 [3]湖南省农业科学院,长沙410125 [4]湖南农业大学农学院,长沙410126

出　　处：《基因组学与应用生物学》2013年第3期353-358,共6页Genomics and Applied Biology

基　　金：农业部转基因专项(2011ZX08001-004;2011ZX08001-001);湖南省自然科学基金(09JJ3046);湖南省农科院科技创新项目(2009hnnkycx17);湖南省科技重大专项(2011FJ1002-2)共同资助

摘　　要：本研究利用差异显示技术检测经低温处理的小麦品种"石新828"及对照材料中的mRNA,获得2条差异片段序列。经Blast比对后,发现其中一条长为293bp的差异片段序列与小麦的一个冷调蛋白基因的mRNA(GenBank:AB097412.1)同源性为99%。该基因编码的蛋白属于磷脂酰乙醇胺结合蛋白家族,命名为wpebp。经荧光定量PCR分析,wpebp基因的表达量随低温处理时间的增长总体呈上升趋势,表明该基因与小麦的抗寒能力相关。We obtained 2 sequences of differential fragment in cold-induced wheat variety ＂Shi Xin 828＂using dif-ferential display method. After Blast analysis, it was indicated that the homology between 293 bp differential frag-ment and mRNA of wheat cold-regulated gene （GenBank： AB097412.1） is 99%. The protein belongs to phos-phatidylethanolamine binding proteins and it was called wpebp. The fluorescence quantitative PCR analysis showed that there was an upward trend in wpebp gene expression quantities as cold-induced time extended. We could con-clude that the gene is related to cold resistance of the wheat.

关 键 词：小麦 mRNA差异显示分析 低温诱导 磷脂酰乙醇胺结合蛋白基因 

分 类 号：S512.1[农业科学—作物学]