胡黄连苷Ⅱ对大鼠脑缺血损伤后NO含量和GSHPx活性的影响  

The effect of picrosideⅡ on the concentration of NO and the activity of GSHPx after cerebral ischemic injury in rats

作　　者：钟亮[1] 张睿[2] 纪晓军[2] 吴艺玲[2] 徐琦[2] 刘洪玲[2] 黄惠[2] 

机构地区：[1]青岛市第三人民医院神经内科,山东青岛266041 [2]青岛大学医学院附属医院ICU,山东青岛266003

出　　处：《中国医药科学》2013年第11期27-29,共3页China Medicine And Pharmacy

基　　金：国家自然科学基金项目(81041092);山东省自然科学基金项目(ZR2011HM050)

摘　　要：目的通过正交试验优化胡黄连苷Ⅱ治疗大鼠脑缺血损伤的最佳剂量和时间窗。方法应用双侧颈总动脉结扎法（BCCAO）建立大鼠前脑缺血模型，按照正交试验设计分组，经腹腔注射胡黄连苷Ⅱ干预治疗，应用硝酸还原酶法测定血清和脑组织中一氧化氮（NO）的含量。化学比色法检测血清和脑组织中谷胱甘肽过氧化物酶（GSHPx）的活性。结果胡黄连苷Ⅱ治疗脑缺血损伤的最佳效果，根据血清和脑组织NO含量分析，均为脑缺血1．5h，腹腔注射20mg，kg体重。根据血清和脑组织GSHPx活性分析，分别为脑缺血1．5h，腹腔注射10mg／k和脑缺血2．0h，腹腔注射20mg／kg体重。结论从用药剂量最小化和治疗时间窗最大化的角度综合评价，胡黄连苷Ⅱ治疗脑缺血损伤的治疗时间窗和剂量最佳组合为脑缺血1．5～2．0h，腹腔注射10-0mg／kg体重。Objective To optimize the therapeutic dose and time window of picrosede II by orthogonal test in cerebral ischemic injury in rats. Methods The forebrain ischemia models were established by bilateral common carotid artery occlusion （BCCAO） methods. The successful models were randomly grouped according to orthogonal experimental design and treated by injecting picroside II intraperitonenally at different ischemic time with different dose. The concentration nitric oxide （NO） in serum and brain tissue were determined by nitratase reduase assay. The activity of glutathione peroxidase （GSHPx） in serum and tissue were determined by chemical colormetry. Results The optimized composition of the therapeutic dose and time window of picroside II in cerebral ischemic injury was （1） ischemia 1.5 h with 20 mg/kg body weight according to the concentrations of NO in serum and brain tissue; （2） ischemia 1.5 h with 10 mg/kg and ischemia 2.0 h with 20 mg/kg body weight according to the activities of GSHPx in serum and brain tissue. Conclusion From the principle of lowest therapeutic dose with longest tome window,the optimized composition of the therapeutic dose and time window of picroside II in cerebral ischemic injury is ischemia 1.5-2.0 h with 10-20 mg/kg body weight.

关 键 词：胡黄连苷Ⅱ 脑缺血 剂量 时间窗 NO GSHPX 大鼠 

分 类 号：R338[医药卫生—人体生理学]