指状拟舟虫诱导牙鲆抗血清免疫球蛋白分析  

Analysis of immunogloblin M (IgM) in serum of Japanese flounder, Paralichthys olivaceus

作　　者：周丽[1] 战文斌[1] 宋微波[1] 俞开康 

机构地区：[1]教育部水产养殖开放试验室,青岛海洋大学山东青岛266003

出　　处：《中国水产科学》2000年第2期28-31,共4页Journal of Fishery Sciences of China

基　　金：国家重点基础研究规划资助项目! (G19990 12 0 0 2 ) ;教育部重点课题资助项目! ( 99184) ;"长江学者奖励计划"资助项目

摘　　要：对引起牙鲆体表溃烂的病原纤毛虫—指状拟舟虫诱导牙鲆免疫反应产生的免疫球蛋白IgM进行分析 ,结果表明 ,病原纤毛虫免疫注射牙鲆 ,可诱导牙鲆发生特异性免疫反应 ,产生抗体。牙鲆抗指状拟舟虫血清的凝集试验 ,发现纤毛虫停止游动并发生虫体聚集 ;抗血清经与标准分子量和鼠IgM单克隆抗体以及对照血清的SDS -PAGE电泳比较分析证明 ,牙鲆抗指状拟舟虫血清免疫球蛋白IgM重链分子量为 710 0 0 ,轻链分子量为 2 30 0 0 ;IgM为四聚体 ,分子量为 752 0 0 0。The immune response of Japanese flounder(Paralichthys olivaceus)against antigen of Paralembus digitiformis was detected. Agglutination test and SDS-PAGE were used respectively to investigate the serum antibody response in flounder injected with frozen-killed ciliates. The sera from immunized fish with the ciliated protozoon, Paralembus digitiformis, immobilized the parasite and caused the parasites agglutinated in vitro by agglutination test. The antiserum was analyzed by SDS-PAGE and proved that IgM had produced in the serum of immunized flounder. The molecular weights of the 2 subunits (H-chain and L-chain) of Japanese flounder IgM were estimated 71 000 and 23 000 respectively, by SDS-PAGE with 2-mercaptoethanol. The molecular weight of Japanese flounder IgM was determined to be 752 000.

关 键 词：牙鲆 指状拟舟虫 纤毛虫 免疫球蛋白 免疫应答 

分 类 号：S943.399.5[农业科学—水产养殖] S942.5[农业科学—水产科学]