高效液相色谱法测定小鼠血清中痂囊腔菌素A的浓度  

Determination serum concentration of elsinochrome A in mice by HPLC

作　　者：屠文莲[1] 赵银[1] 王宇[1] 夏爱博[1] 赵刚[1] 

机构地区：[1]云南省第一人民医院,云南昆明650032

出　　处：《中国新药与临床杂志》2013年第6期486-489,共4页Chinese Journal of New Drugs and Clinical Remedies

基　　金：云南省自然科学基金资助项目(2000C0106M)

摘　　要：目的建立高效液相色谱（HPLC）法测定小鼠血清中痂囊腔菌素A的浓度，考察其在小鼠体内的药动学特征。方法60只小鼠，雌雄各半，痂囊腔菌素A30．0mg灌胃后，于不同时间点分取血浆，采用HPLC分别测定血清样品中痂囊腔菌素A浓度，用DAS3．0．8软件计算药动学参数。结果痂囊腔菌素A在10．0～800．0μg·L-1内线性关系良好（r=0．9996），日内和日间RSD〈7．0％，提取回收率为89-2％～92．4％，方法回收率为100．1％～100．6％。痂囊腔菌素A在小鼠体内的主要药动学参数：AUC。匀（16697．8-4-873．0）μg-L·h-1，MRTo。为（3．8±0．0）h，：为（3．6±0．0）h，为（1．0±0．0）h，CLz／F为（1．8±0．0）L-h，p-1为（5889．3±135．9）μg·L-1。结论本法简便、灵敏、重现性好。痂囊腔菌素A在小鼠体内药动学过程符合二室模型。AIM To establish a HPLC method for determining the serum concentration of elsinochrome A and to investigate its pharmacokinetic in mice. METHODS After the plasma from 60 mice （half male and half female） with intragastric were collected in different time point, elsinochrome A in serums were collected and determined by HPLC. The pharmacokinetic parameters were calculated by DAS 3.0.8. RESULTS The good linear range of elsinochrome A were from 10.0 - 800.0 gL-1（r = 0.999 6）. The limit of quantitation （LOQ） was 1.0 g.L-1. The RSD （%） of intra-and inter-day were all below 〈 7.0%. The extraction recoveries were in the range of 89.2% - 92.4% and the method recoveries were 100.1% - 100.6%. The main pharmacokinetic parameters of mice were as follows： AUC0 （16 697.8 ±873.0） μg.Lh-1, MRT0 = （3.8 ± 0.0） h,z = （3.6 ± 0.0） h, t,r = （1.0 ±0.0） h, CLz/F= （1.8 ± 0.0） L.h-1, p = （5 889.3 ± 135.9） μg.L-1. CONCLUSION This method is convenient, sensitive and reproducible. The pharmacokinetic character of Elsinochrome A in mouse after oral showes linear and conforms to the two compartment models.

关 键 词：痂囊腔菌素A 小鼠 色谱法 高压液相 药动学 

分 类 号：R285.5[医药卫生—中药学]