婚飞行为影响中华蜜蜂性成熟处女蜂王的基因表达  

Mating flight behaviour affects gene expression in matured virgin queens of Apis cerana cerana (Hymenoptera: Apidae)

作　　者：吴小波[1] 王子龙[1] 张飞[1] 石元元[1] 曾志将[1] 

机构地区：[1]江西农业大学蜜蜂研究所

出　　处：《昆虫学报》2013年第5期486-493,共8页Acta Entomologica Sinica

基　　金：国家自然科学基金项目(31060327);江西省研究生创新基金项目(YC2011-B016);江西农业大学青年科学基金项目(2010-09003303)

摘　　要：婚飞是性成熟处女蜂王与雄蜂交配过程中的一个重要前奏,在该过程中蜂王体内伴随着一系列重要的生理变化。为了探究中华蜜蜂Apis cerana cerana处女蜂王婚飞过程中基因表达变化,本研究利用数字基因表达谱(digital gene expression,DGE)技术分析了中华蜜蜂性成熟处女蜂王飞行与未飞行之间的基因表达差异。经DGE测序,分别从两个样品中获得5.98和6.01百万条Clean标签。通过分析检测到250个基因有差异表达,其中133个基因在飞行蜂王中上调表达,117个基因在飞行蜂王中下调表达。这些差异基因可以归类到348个功能性类别和142个生化途径。结果表明中华蜜蜂性成熟处女蜂王在婚飞过程中大量基因的表达发生了变化。这些结果为进一步研究中华蜜蜂蜂王婚飞过程中生理变化的分子机制提供了重要的基因表达信息。Queen mating flight is an important prelude of sexual matured virgin queens mating with drones, which is usually accompanied by a series of important physiological changes in queens＇ bodies. In order to explore the differences of gene expression following queen mating flight behaviour, we analyzed the gene expression differences between the flying matured virgin queens and non-flying matured virgin queens of Apis cerana cerana using a high-throughput sequencing method. Through digital gene expression （DGE） sequencing, we obtained 5.98 and 6.01 million clean tags from the flying matured virgin queen and non-flying matured virgin queen samples, respectively. A total of 250 genes were differentially expressed between both, with 133 up-regulated and 117 down-regulated in the flying matured virgin queens. These differentially expressed genes can be classified into 348 functional categories and involved in 142 biochemical pathways, indicating that there are a large number of genes whose expression levels change during mating flight process of matured virgin queens. The results provide important gene expression information for further researching the molecular mechanisms of the physiological changes during queen mating flight in A. cerana cerana.

关 键 词：中华蜜蜂 蜂王 飞行行为 数字基因表达谱 基因表达 婚飞 

分 类 号：Q966[生物学—昆虫学]