细菌感染后家蚕幼虫中肠差异表达基因的鉴定(英文)  

Identification of differentially expressed genes in the larval midgut of the silkworm (Bombyx mori) after bacterial infection

作　　者：朱艳菊[1] 吕志强[1,2] 

机构地区：[1]西北农林科技大学植物保护学院昆虫学系,陕西杨凌712100 [2]西北农林科技大学植保资源与病虫害治理教育部重点实验室,陕西杨凌712100

出　　处：《昆虫学报》2013年第5期505-511,共7页Acta Entomologica Sinica

基　　金：国家重大基础研究发展规划("973"计划)项目子课题(2012CB114604);教育部基本科研业务费专项资金(CX200908)

摘　　要：作为遭遇各种微生物的前线,昆虫的消化道在免疫反应中起到重要的作用。为了研究家蚕Bombyx mori肠道免疫反应,我们利用基于ACP(annealing control primer)的反转录PCR技术,从中肠组织中鉴定得到18个在经口器感染绿脓杆菌Pseudomonas aeruginosa和金黄色葡萄球菌Staphylococcus aureus后的差异表达基因,并利用荧光定量PCR分析了其中4个基因在感染后24h内的动态变化。结果表明:肽聚糖识别蛋白-L1(PGRP-L1)和一个丝氨酸蛋白酶前体基因特异性地受绿脓杆菌感染后上调;30kP蛋白酶A基因受绿脓杆菌和金黄色葡萄球菌2种细菌感染后上调。我们的研究鉴定了经口器感染后家蚕中肠中参与入侵细菌识别和免疫信号途径的基因,可为对这些基因进一步的功能研究提供线索。As the frontline encountering various microbes, intestine plays critical roles in insect immune responses. In order to investigate the immune responses in the silkworm midgut, we identified 18 differentially expressed genes after oral infection by Pseudomonas aeruginosa and Staphylococcus aureus using annealing control primer （ACP） based reverse transcriptional PCR technique. The expression profile of four genes during the first 24 hours post infection was monitored by quantitative real-time PCR. The results showed that the peptidoglycan recognition protein L1 （PGRP-L1） gene and a serine protease precursor gene were up-regulated by P. aeruginosa infection exclusively, while the 30kP protease A precursor gene was up-regulated significantly by both P. aeruginosa and S. aureus infection. Our study identified genes involved in recognition of invading bacteria and immune signaling pathways in the larval midgut of the silkworm after oral infection and may give some clues for further functional investigation of these ~enes.

关 键 词：家蚕 中肠 细菌感染 免疫反应 差异表达基因 

分 类 号：Q966[生物学—昆虫学]